Related Experiment Video
Updated: Jan 10, 2026
Establishment of an Experimental Mouse Model of Endometrioma to Study its Related Infertility
Published on: April 5, 2024
Identifying serum microRNAs as biomarkers for endometriosis in adolescents and young adults
Alla Vash-Margita1, Ramanaiah Mamillapalli2, Karthik Pyneni3
1Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, 310 Cedar Street, New Haven, CT, 06520, USA. alla.vash-margita@yale.edu.
Background:
Endometriosis is a gynecological disorder that affects 190 million reproductive age women worldwide. Laparoscopic surgery is considered the gold standard to diagnose the disease, creating a barrier to diagnosis and leading to long delays in disease recognition. MicroRNAs may be useful in the diagnosis of this disease, however the ability to detect early disease in adolescents may be improved by identifying microRNAs specific to this population.
Methods:
This was a prospective clinical study evaluating adolescent patients with pelvic pain undergoing gynecologic surgery in an academic medical center. We enrolled 63 adolescent and young adult patients aged 13-26 years old undergoing gynecologic surgery between 2019 and 2024. Clinically relevant phenotypic and surgical information were recorded as well as evaluation of microRNAs abundance. We assessed microRNAs abundance by extracting total RNA from the serum samples and performed RNA-sequencing (RNAseq).
Results:
The mean age of adolescent women in the study was 16.3 and 15.9 for the endometriosis (n = 31) and control groups (n = 20), respectively. The mean BMI was 24.5 kg/m2 and 29.0 kg/m2 in the endometriosis and control groups, respectively. RNA-seq data analysis showed differential abundance of 859 microRNAs. Among 859 microRNAs, 488 were increased and 391 were decreased. We next selected those that were most distinct, with little overlap between subjects and controls. Four microRNAs were highly significantly increased while eighteen microRNAs were highly significantly decreased. We defined a signature of microRNAs that best distinguished subjects with endometriosis from controls.
Conclusions:
This is the first study to reveal the differential abundance of microRNAs specifically in adolescent patients with endometriosis. There are distinct differences from those identified in adult women with endometriosis. Our findings present a unique signature of microRNA found in the serum of adolescents with endometriosis. This finding may be useful as a noninvasive biomarker to diagnose early disease in adolescents. Non-invasive diagnosis may allow for early diagnosis and prevention of disease progression.
