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Identification and Functional Characterization of Salt-Tolerant Long Non-Coding RNAs in Tamarix hispida.

Xin Xu1,2, Lei Li1,2, Zhibo Wang3

  • 1College of Forestry, Shenyang Agricultural University, Shenyang, China.

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Summary

This study identifies five salt-responsive long non-coding RNAs (lncRNAs) in Tamarix hispida that enhance salt tolerance. Overexpression of these lncRNAs, particularly ThSAIR5, improves stress response and proline production, offering potential for crop improvement.

Keywords:
Tamarix hispidaThSAIR5long non‐coding RNAreactive oxygen species (ROS) scavengingsalt tolerance

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Area of Science:

  • Plant Molecular Biology
  • Genetics and Genomics
  • Stress Physiology

Background:

  • Long non-coding RNAs (lncRNAs) are crucial regulators of plant growth, development, and responses to abiotic stresses like salinity.
  • Understanding lncRNA functions in stress-tolerant species is vital for improving crop resilience.

Purpose of the Study:

  • To identify and functionally characterize salt-responsive lncRNAs in Tamarix hispida roots and leaves.
  • To investigate the role of specific lncRNAs (ThSAIR1-ThSAIR5) in enhancing salt tolerance.
  • To elucidate the molecular mechanism underlying ThSAIR5-mediated salt tolerance.

Main Methods:

  • RNA sequencing (RNA-seq) to identify salt-induced lncRNAs and their target genes.
  • Agrobacterium-mediated transient transformation for overexpression of ThSAIR1-ThSAIR5 in T. hispida.
  • Analysis of physiological parameters (ROS scavenging, proline biosynthesis) under salt stress.
  • Transcriptome sequencing and gene transformation in Arabidopsis thaliana to validate target gene function.

Main Results:

  • 1112 salt-induced lncRNAs and 7198 mRNAs were identified in T. hispida under salt stress.
  • Transient overexpression of ThSAIR1-ThSAIR5 enhanced reactive oxygen species (ROS) scavenging and proline biosynthesis.
  • ThSAIR5 was identified as a positive regulator of salt tolerance, acting through its target gene ThNAC86.
  • Transgenic Arabidopsis thaliana overexpressing ThNAC86 exhibited improved salt tolerance.

Conclusions:

  • ThSAIR1-ThSAIR5 are key lncRNAs involved in T. hispida's salt stress response.
  • ThSAIR5 enhances salt tolerance by positively regulating ThNAC86 expression.
  • These findings highlight the potential of ThSAIR5 and ThNAC86 as candidate genes for developing salt-tolerant crops through molecular breeding.