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A Method of Targeted Cell Isolation via Glass Surface Functionalization

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

Surface Engineered Biomolecular Condensates for Targeted Cell Cytotoxicity.

Chengying Yin¹, Cheng Wu^2,3, Xinran Yu⁴

¹Department of Ambulatory Surgery, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310027, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|November 25, 2025

Summary

This summary is machine-generated.

Engineered biomolecular condensates with a decanoic acid membrane precisely control enzyme location and activity. This enables targeted drug delivery and enhanced biocatalysis, showing potential for synthetic organelles in therapeutics.

Keywords:

biomolecular condensates cell cytotoxicity enzymatic reaction surface engineering

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Area of Science:

Biomolecular engineering
Synthetic biology
Biocatalysis

Background:

Biomolecular condensates are key for cellular organization but lack precise interfacial control.
Open interfaces hinder enzymatic activity and targeted applications.

Purpose of the Study:

To develop a strategy for interfacial engineering of biomolecular condensates.
To achieve precise spatial control over biomacromolecules and modulate enzymatic activity.
To demonstrate the therapeutic potential of engineered condensates for targeted delivery and cell apoptosis.

Main Methods:

A two-step interfacial engineering strategy to form a decanoic acid membrane on decalysine/polyinosinic acid condensates.
Investigated molecular-weight-dependent spatial localization of biomacromolecules (≤60 kDa vs. high-molecular-weight).
Assessed modulated enzymatic kinetics (lipase, alkaline phosphatase) and in vitro cell co-culture delivery of alkaline phosphatase.

Main Results:

Decanoic acid membrane formation reduced interfacial mobility and enhanced hydrophobic molecule enrichment.
Achieved spatial control of biomacromolecules based on molecular weight, localizing smaller proteins inside and larger ones at the interface.
Demonstrated enhanced catalytic activity of interfacial alkaline phosphatase, leading to triggered release of ssDNA and significant reduction in HeLa cell viability (to 5%).

Conclusions:

Interfacial engineering of biomolecular condensates offers precise control over molecular localization and enzymatic function.
Engineered condensates serve as effective platforms for targeted biocatalysis and therapeutic delivery.
This approach enables functional customization of synthetic organelles for advanced biological applications.