Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

97.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.6K
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

4.6K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
4.6K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

554
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
554
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

12.6K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
12.6K
RNA-seq03:21

RNA-seq

11.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.7K
Sanger Sequencing01:57

Sanger Sequencing

772.9K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
772.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Study on the diversity, structure, and function of endophytic bacteria in seeds of genuine medicinal plants in gansu province.

BMC plant biology·2026
Same author

MMSpa is a deep learning-based tool that enhances the identification of spatial domains in spatial transcriptomics studies.

PLoS biology·2026
Same author

HAlign-G: rapid and low-memory multiple-genome aligner for large-scale closely related genomes.

Genome biology·2025
Same author

ReAlign-P: a vertical iterative realignment method for protein multiple sequence alignment.

Bioinformatics (Oxford, England)·2025
Same author

FORAlign: accelerating gap-affine DNA pairwise sequence alignment using FOR-blocks based on Four Russians approach with linear space complexity.

Briefings in bioinformatics·2025
Same author

ReAlign-N: an integrated realignment approach for multiple nucleic acid sequence alignment, combining global and local realignments.

NAR genomics and bioinformatics·2024
Same journal

Novel variants in LSS related hypotrichosis simplex 14.

Frontiers in genetics·2026
Same journal

Network-based analysis identifies shared mechanisms between ischemic stroke and myocardial infarction and therapeutic ingredients of Buyang Huanwu Decoction.

Frontiers in genetics·2026
Same journal

GWAS analysis of a depression cohort defined by an EHR-phenotyping algorithm reveals the role of immune regulations in depression risk.

Frontiers in genetics·2026
Same journal

Ferroptosis, lipid metabolism, and genetic regulation in postoperative rehabilitation of elderly hip fractures: from molecular mechanisms to clinical translation.

Frontiers in genetics·2026
Same journal

Single-cell and pseudobulk analyses reveal hidden mitochondrial expression imbalance in gastric cancer.

Frontiers in genetics·2026
Same journal

Transcriptomic profiling and experimental validation of myeloid-cell-differentiation-related key genes in osteoarthritis.

Frontiers in genetics·2026
See all related articles

Related Experiment Video

Updated: Jan 10, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

3.9K

Advances in post-processing methods for multiple sequence alignment.

Yixiao Zhai1,2, Zitong Zhang3, Zhen Li2

  • 1Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu, China.

Frontiers in Genetics
|November 25, 2025
PubMed
Summary
This summary is machine-generated.

Multiple sequence alignment (MSA) quality is crucial for biological research reliability. This review systematically examines MSA post-processing optimization methods developed over 30 years to improve alignment accuracy.

Keywords:
meta-alignmentmultiple sequence alignmentpost-processingrealignerrealignment

More Related Videos

A Practical Guide to Phylogenetics for Nonexperts
12:00

A Practical Guide to Phylogenetics for Nonexperts

Published on: February 5, 2014

36.0K
Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin
08:57

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Published on: August 14, 2018

16.4K

Related Experiment Videos

Last Updated: Jan 10, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

3.9K
A Practical Guide to Phylogenetics for Nonexperts
12:00

A Practical Guide to Phylogenetics for Nonexperts

Published on: February 5, 2014

36.0K
Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin
08:57

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Published on: August 14, 2018

16.4K

Area of Science:

  • Bioinformatics
  • Computational Biology
  • Genomics

Background:

  • Multiple Sequence Alignment (MSA) is fundamental for biological research.
  • MSA is an NP-hard problem, making globally optimal solutions unattainable.
  • Current research focuses on improving MSA accuracy through post-processing optimization.

Purpose of the Study:

  • To systematically review the development of MSA post-processing methods over the last three decades.
  • To summarize key research ideas and advancements in MSA alignment accuracy.
  • To identify future research directions in MSA optimization.

Main Methods:

  • Systematic literature review of MSA post-processing techniques.
  • Analysis of algorithmic developments and optimization strategies.
  • Synthesis of research trends over a 30-year period.

Main Results:

  • Documented evolution of MSA post-processing methodologies.
  • Identified key strategies for enhancing alignment quality.
  • Highlighted the increasing importance of optimization in MSA.

Conclusions:

  • Post-processing optimization is a vital strategy for improving MSA reliability.
  • A comprehensive understanding of past developments is essential for future research.
  • Further investigation into novel post-processing techniques is warranted for advancing bioinformatics.