Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A sensitive orange fluorescent calcium ion indicator for imaging neural activity.

Nature communications·2026
Same author

Intramedullary screw fixation of ballistic metacarpal and phalanx fractures.

JPRAS open·2026
Same author

Exploring technology-delivered cognitive training in the perioperative period for the prevention of postoperative delirium: a scoping review.

Psychology, health & medicine·2026
Same author

Modulation of miR-23b Wnt/β-catenin Axis Strengthens Endothelial Barrier Properties.

bioRxiv : the preprint server for biology·2026
Same author

Reduced SH3RF3 May Protect Against Alzheimer's Disease by Lowering Microglial Pro-Inflammatory Responses via Modulation of JNK and NFkB Signaling.

Glia·2026
Same author

High prevalence of CNS-directed autoantibodies in patients with schizophrenia.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: May 8, 2026

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
08:14

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

Published on: December 3, 2015

7.9K

Generation of hiPSC-Derived Brain Microvascular Endothelial Cells Using Directed Differentiation and Transcriptional

Aomeng Cui1, Ronak Patel2, Patrick Bosco2

  • 1Department of Neurology (A.C., U.A., D.A.), Columbia University Irving Medical Center, New York, NY.

Arteriosclerosis, Thrombosis, and Vascular Biology
|November 25, 2025
PubMed
Summary

Researchers developed reprogrammed brain microvascular endothelial cells (rBMECs) from induced pluripotent stem cells (iPSCs) to better model the human blood-brain barrier (BBB). These rBMECs exhibit enhanced BBB properties and a brain-specific transcriptome, aiding Alzheimer disease research.

Keywords:
astrocytesblood-brain barrierendothelial cellspericytestight junctions

More Related Videos

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition
09:00

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Published on: June 16, 2019

10.0K
Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells
04:23

Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

Published on: March 31, 2021

2.6K

Related Experiment Videos

Last Updated: May 8, 2026

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
08:14

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

Published on: December 3, 2015

7.9K
Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition
09:00

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Published on: June 16, 2019

10.0K
Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells
04:23

Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

Published on: March 31, 2021

2.6K

Area of Science:

  • Neuroscience
  • Stem Cell Biology
  • Vascular Biology

Background:

  • Modeling the human blood-brain barrier (BBB) is challenging due to limitations in generating functional induced pluripotent stem cell (iPSC)-derived brain microvascular endothelial cells (BMECs).
  • Existing methods fail to fully replicate key BMEC functions and the specific gene expression patterns of brain endothelial cells.

Purpose of the Study:

  • To develop a robust protocol for generating reprogrammed BMECs (rBMECs) with enhanced blood-brain barrier properties using iPSCs.
  • To investigate the impact of familial Alzheimer disease mutations on BBB integrity and inflammatory responses in a 3D neurovascular model.

Main Methods:

  • Directed differentiation of human iPSCs combined with overexpression of transcription factors FOXF2 and ZIC3 to create rBMECs.
  • Characterization using immunofluorescence, functional assays, and bulk RNA sequencing.
  • Co-culture of rBMECs with iPSC-derived astrocytes and pericytes in a microfluidic platform to create a 3D neurovascular unit.

Main Results:

  • rBMECs demonstrated a subset of the BBB transcriptome, improved barrier function, reduced transcytosis, and comparable P-glycoprotein activity to primary BMECs.
  • The 3D neurovascular system formed by rBMECs, astrocytes, and pericytes exhibited robust BBB properties.
  • Familial Alzheimer disease mutation (APP V717I) in rBMECs led to decreased barrier integrity and increased inflammatory markers, distinct from oligomeric amyloid-β effects.

Conclusions:

  • The developed protocol successfully generates rBMECs with significant BBB properties and a brain-specific transcriptome.
  • The iPSC-derived 3D neurovascular unit model effectively recapitulates aspects of the in vivo human BBB.
  • The APP V717I mutation independently affects BBB properties and inflammatory status, offering insights into Alzheimer disease pathogenesis.