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Updated: Jan 10, 2026

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

Molecularly Engineered Dual-Lock Activatable NIR-II Fluorogenic Probe for Precision Tumor Resection and Real-Time

Jiao Lu^1,2, Xinyu Song³, Xunkai Wang^1,2

¹NHC Key Laboratory of Tropical Disease Control, Engineering Research Center for Hainan Bio-Smart Materials and Bio-Medical Devices, Key Laboratory of Hainan Functional Materials and Molecular Imaging, School of Life Sciences and Medical Technology, Hainan Medical University, Haikou, Hainan 571199, China.

Analytical Chemistry

|November 25, 2025

Summary

This summary is machine-generated.

We developed novel fluorescent probes for real-time cancer surgery. These probes precisely visualize tumor margins and monitor treatment effectiveness, improving precision oncology outcomes.

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Published on: July 17, 2012

Area of Science:

Oncology
Biomedical Engineering
Molecular Imaging

Background:

Complete surgical resection is crucial for solid tumor treatment.
Intraoperative tumor margin visualization and real-time response monitoring remain clinical challenges.

Purpose of the Study:

To engineer novel dual-activatable near-infrared-II (NIR-II) fluorescent probes.
To enable simultaneous detection of tumor malignancy and therapeutic response.

Main Methods:

Designed and synthesized a series of tandem-locked, dual-activatable NIR-II fluorescent probes (HY-1-NO2 to HY-4-NO2).
Evaluated probe performance based on response to nitroreductase (NTR) and microenvironmental viscosity.
Assessed probe efficacy in delineating tumor margins and monitoring therapeutic response in preclinical models and clinical specimens.

Main Results:

HY-2-NO2 exhibited a 20-fold fluorescence enhancement in the NIR-II window (910 nm) upon dual activation.
Achieved precise intraoperative tumor margin delineation with accuracy down to 0.2 mm.
Demonstrated real-time monitoring of paclitaxel treatment efficacy, with fluorescence intensity correlating positively with tumor suppression.
Successfully differentiated tumor from normal tissues in clinical specimens.

Conclusions:

Developed an integrated theranostic platform for real-time surgical navigation and functional treatment monitoring.
The dual-activatable probe addresses critical needs in precision oncology.
Demonstrated strong translational potential for improved cancer care.