Related Experiment Video
Updated: Jan 10, 2026

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
Published on: January 5, 2024
Development of two compatible plasmids to assess sRNA-mediated post-transcriptional regulation in Acinetobacter
Aalap Mogre1, Orla Connell1, Jessica White1
1Department of Microbiology School of Genetics & Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin, Ireland.
Abstract:
Post-transcriptional regulation can be mediated by small regulatory RNAs (sRNAs) in bacteria, which can act by base-pairing to a target mRNA. The discovery and mechanistic validation of base-pairing sRNAs in multidrug-resistant Acinetobacter baumannii has been hampered by the lack of genetic tools to assess RNA-RNA interactions. Here, we created two compatible plasmids for A. baumannii, which addresses this need. The newly designed plasmids validated the known Aar sRNA-carO mRNA and a new interaction of sRNA44 and the mRNA of the biofilm-associated protein Bap. The new plasmid system should accelerate the mechanistic characterization of sRNAs in A. baumannii.
Related Concept Videos
Antibiotic Selection
Translational Regulation
Coordination of Gene Expression Processes in Bacteria

