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A Murine Model of a Burn Wound Reconstructed with an Allogeneic Skin Graft
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Fibroblast Dynamics Following Partial and Deep Burn Injury in a Reconstructed Human Skin Model.

Britt van der Leeden1,2, H Ibrahim Korkmaz2,3,4,5, Sanne Roffel6

  • 1Department of Pathology, Amsterdam UMC Location Vrije Universiteit Amsterdam, Boelelaan 1117, Amsterdam, The Netherlands.

Tissue Engineering and Regenerative Medicine
|November 25, 2025
PubMed
Summary
This summary is machine-generated.

A new 3D reconstructed human skin (RhS) model effectively mimics burn injuries, revealing fibroblast migration and phenotype changes crucial for understanding wound healing and developing future burn therapies.

Keywords:
Fibroblast activating proteinLactate dehydrogenasePapillary fibroblastsPhosphorylated ribosomal protein S6

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Area of Science:

  • Biotechnology
  • Wound Healing Research
  • Tissue Engineering

Background:

  • Burn injuries cause prolonged inflammation, hindering wound healing and increasing scarring risk.
  • Understanding fibroblast behavior post-burn is vital for improving patient recovery.
  • Current models lack the complexity to fully study burn pathophysiology.

Purpose of the Study:

  • To develop a 3D reconstructed human skin (RhS) model for burn injuries.
  • To mimic superficial, partial-thickness, and deep burn injuries.
  • To assess fibroblast dynamics and behavior in the RhS model over one week.

Main Methods:

  • Constructed RhS with a reconstructed epidermis on a fibroblast-populated collagen hydrogel dermis.
  • Induced burns of varying temperatures (70°C, 110°C, 140°C) in the RhS model.
  • Analyzed RhS up to one week post-burn using histological staining and protein analysis.

Main Results:

  • Distinct histological zones (viable, mixed, necrotic) were observed in partial and deep burn models.
  • Demonstrated fibroblast migration from wound edges and increased papillary fibroblast markers (FAP+).
  • Burn temperature significantly influenced inflammatory and tissue remodeling mediator secretion (e.g., SAA, MMP-9).

Conclusions:

  • The RhS burn model provides an organotypic platform to study complex post-burn fibroblast dynamics.
  • This model facilitates research into burn pathophysiology.
  • It serves as a valuable tool for evaluating future therapeutic strategies to enhance burn wound healing and minimize scarring.