Related Experiment Video

Updated: Jun 28, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

14.4K

Functional interrogation of contextually-correct MYH7 variants using CRaTER-flox geneediting and contractility

Alexander M Loiben1, Wei-Ming Chien1, Ashley McKinstry1

  • 1Department of Medicine/Cardiology, University of Washington, Seattle, United States of America.

The Journal of Clinical Investigation
|November 25, 2025
PubMed
Summary

No abstract available in PubMed .

Keywords:
CardiologyCardiovascular diseaseGenetic diseasesGeneticsHeart failure

More Related Videos

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.9K
Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing
10:12

Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

Published on: June 16, 2022

4.7K

Related Experiment Videos

Last Updated: Jun 28, 2026

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

14.4K
Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.9K
Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing
10:12

Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

Published on: June 16, 2022

4.7K

Related Concept Videos

Point and Frameshift Mutations01:30

Point and Frameshift Mutations

Point mutations are genetic alterations involving the change of a single nucleotide base pair in DNA. Depending on how the alteration affects protein synthesis, they can lead to various consequences.Point mutations fall into the following types:Silent mutations occur when a nucleotide change does not alter the amino acid sequence due to the redundancy of the genetic code. For instance, changing ACC to ACA still encodes threonine, leaving the protein function unaffected. This occurs because...

Articles linked to this work by shared authors, journal, and citation graph.

Contractility Drives Cardiomyocyte Maturation and the Response to Nanopatterns.

Circulation research·2026

The E525K β-Myosin Mutation Causes Hypocontractility in Cardiomyocytes Without Altering Loaded Crossbridge Cycling.

bioRxiv : the preprint server for biology·2026

The hypertrophic cardiomyopathy mutation G768R makes cardiac myosin a high duty ratio motor.

bioRxiv : the preprint server for biology·2026

Circumferential strain recovery after human cardiomyocyte transplantation in minipigs using a novel frequency-based method for myocardial tagging quantification.

Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance·2026

Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effects.

Cell·2026

The hypertrophic cardiomyopathy myosin variant G256E prolongs cardiac muscle relaxation via altered nucleotide handling.

Communications chemistry·2026

Cholesterol-responsive NFE2L1-INSIG1 interaction controls VLDL secretion and metabolic dysfunction-associated steatohepatitis pathogenesis in mice.

The Journal of clinical investigation·2026

The splice of life: an isoform-centric view of disease, technology, and therapeutics.

The Journal of clinical investigation·2026

Histone lysine methyltransferases KMT2C and KMT2D join the all-star tumor suppressor team in gastrointestinal cancer.

The Journal of clinical investigation·2026

Targeting hepatic cholesterol sensing to tackle metabolic dysfunction-associated steatohepatitis.

The Journal of clinical investigation·2026

Gut microbe-derived short-chain fatty acids regulate alphavirus arthritis and macrophage activation in mice.

The Journal of clinical investigation·2026

Sterol biosynthesis, brain development, and disease.

The Journal of clinical investigation·2026

Genomic landscape of Mexican patients with maturity onset diabetes of the young: beyond mutations in MODY-known genes.

Frontiers in endocrinology·2026

Linking <i>SIRT1</i> gene variation and protein levels to the pathophysiology of type 2 diabetes.

Molecular biology research communications·2026

Pharmacogenetic analyses in people with dementia in Northeast Germany.

Alzheimer's & dementia (Amsterdam, Netherlands)·2026

Research progress on targeted regulatory proteins in the prevention and treatment of atherosclerosis.

Frontiers in immunology·2026

Retraction Note: Micropropagation and assessment of genetic fidelity of <i>Dendrocalamus strictus</i> (Roxb.) nees using RAPD and ISSR markers.

3 Biotech·2026

Distribution of ST116 carbapenem-resistant <i>Citrobacter freundii</i> in public genomes and characterization of a triple-carbapenemase-producing strain.

Frontiers in cellular and infection microbiology·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us