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Related Experiment Videos

Spheroid chromatin units (v bodies).

A L Olins, D E Olins

    Science (New York, N.Y.)
    |January 25, 1974
    PubMed
    Summary

    Researchers observed linear arrays of spherical chromatin particles, termed nu bodies, approximately 70 angstroms in diameter. These structures, with connecting strands, were found in isolated eukaryotic nuclei from various animal tissues.

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    Osmium ammine-B and electron spectroscopic imaging of ribonucleoproteins: correlation of stain and phosphorus.

    Biology of the cell·1996

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • Eukaryotic DNA is organized into chromatin within the nucleus.
    • The higher-order structure of chromatin influences gene regulation and nuclear processes.

    Purpose of the Study:

    • To investigate the ultrastructural organization of chromatin in isolated eukaryotic nuclei.
    • To identify and characterize the basic structural units of chromatin.

    Main Methods:

    • Isolation of nuclei from rat thymus, rat liver, and chicken erythrocytes.
    • Swelling nuclei in water.
    • Centrifugation of nuclei onto carbon films.
    • Positive and negative staining techniques for electron microscopy.

    Main Results:

    • Observation of linear arrays of spherical chromatin particles (nu bodies) with a diameter of approximately 70 angstroms.
    • Identification of connecting strands, about 15 angstroms wide, between adjacent nu bodies.
    • Consistent observation of these structures across different eukaryotic cell types.

    Conclusions:

    • Eukaryotic chromatin exists in ordered linear arrays of nu bodies.
    • These findings provide insights into the fundamental structural organization of chromatin at the nanoscale.
    • The observed structure may represent a key level of chromatin packing.

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