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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jan 6, 2026

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Flow Cytometry: a versatile tool for stem cell research.

Sonal M Manohar¹, Shriya Chaubal¹, Srabani Mukherjee²

¹Department of Biological Sciences, Sunandan Divatia School of Science, SVKM's NMIMS (Deemed-to-be) University, Vile Parle (West), Mumbai, Maharashtra, India.

Biochemical and Biophysical Research Communications

|November 25, 2025

Summary

This summary is machine-generated.

Flow cytometry and fluorescence-activated cell sorting (FACS) enable precise stem cell identification and characterization. These methods offer high-throughput, single-cell resolution for analyzing stem cell markers and isolating rare populations.

Keywords:

Flow cytometry Intracellular staining Stem cell characterization Stem cells Surface markers

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Last Updated: Jan 6, 2026

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

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Published on: December 18, 2014

Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods

Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods

Published on: November 19, 2019

Area of Science:

Biotechnology
Cell Biology
Stem Cell Research

Background:

Stem cell identification requires analyzing specific marker expression.
Bulk techniques like qRT-PCR and Western blotting lack single-cell resolution.
Immunofluorescence provides qualitative single-cell data.

Purpose of the Study:

To review the application of flow cytometry in stem cell identification and characterization.
To highlight recent advances and approaches in flow cytometry for stem cell studies.
To discuss the advantages of flow cytometry over traditional methods.

Main Methods:

Flow cytometry for single-cell analysis of marker expression.
Fluorescence-activated cell sorting (FACS) for isolating specific cell populations.
Quantitative analysis of cell surface and intracellular markers.

Main Results:

Flow cytometry provides high-throughput, quantitative, single-cell resolution for stem cell analysis.
FACS enables the physical isolation of rare stem cell populations.
Flow cytometry allows simultaneous assessment of marker expression and cell proliferation.

Conclusions:

Flow cytometry is a powerful tool for identifying and characterizing diverse stem cell types.
Recent advances enhance the utility of flow cytometry in stem cell research.
Flow cytometry and FACS are crucial for advancing stem cell biology and applications.