Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>In Situ</i> Kinetics of Solution-Phase Biomolecular Reactions and Interactions through Single-Molecule Displacement Statistics.

ACS central science·2026
Same author

Electronic origin of reorganization energy in interfacial electron transfer.

Nature·2026
Same author

High-Purity Monovalent Functionalization of Carbon Nanotubes.

Angewandte Chemie (International ed. in English)·2026
Same author

Class-I myosin responds to changes in membrane tension during clathrin-mediated endocytosis in human induced pluripotent stem cells.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Label-Free Interference Microscopy and Single-Molecule Displacement Mapping Elucidate the Microscopic Structure and Dynamics of Stacked Lipid Bilayers.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Class-I myosin responds to changes in membrane tension during clathrin-mediated endocytosis in human induced pluripotent stem cells.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jan 10, 2026

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
07:53

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Published on: January 16, 2018

8.7K

Direct microsecond wide-field single-molecule tracking and super-resolution mapping via CCD vertical shift.

Megan A Steves1, Ke Xu2

  • 1Department of Chemistry, University of California, Berkeley, Berkeley, California, USA.

Nature Communications
|November 25, 2025
PubMed
Summary
This summary is machine-generated.

SpeedyTrack enables microsecond wide-field single-molecule tracking by using EM-CCD cameras to project time into space. This method achieves high temporal resolution for fast-diffusing molecules and super-resolution mapping in live cells.

More Related Videos

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.7K
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

14.4K

Related Experiment Videos

Last Updated: Jan 10, 2026

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
07:53

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Published on: January 16, 2018

8.7K
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.7K
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

14.4K

Area of Science:

  • Biophysics
  • Optical Imaging
  • Nanotechnology

Background:

  • Wide-field single-molecule tracking is crucial for understanding molecular dynamics.
  • Current methods are limited by camera frame times, hindering the observation of fast molecular motion.

Purpose of the Study:

  • To develop a novel technique for microsecond-wide-field single-molecule tracking and imaging.
  • To overcome the temporal resolution limitations of conventional camera-based tracking.

Main Methods:

  • SpeedyTrack utilizes the vertical charge shifting capability of EM-CCDs to stagger images spatially.
  • This effectively projects the time domain onto the spatial domain of the CCD chip.
  • VS-SpeedyTrack further deconvolves spatial and temporal information for super-resolution mapping.

Main Results:

  • Achieved wide-field tracking of molecules diffusing up to 1000 µm²/s with 50 µs temporal resolution.
  • Demonstrated concurrent Förster resonance energy transfer measurements for molecular state elucidation.
  • Resolved the diffusion mode of a fluorescent protein in live cells with nanoscale resolution.

Conclusions:

  • SpeedyTrack provides a facile solution for microsecond single-molecule tracking without hardware modifications.
  • Enables super-resolution mapping of molecular trajectories in the wide field.
  • Opens new avenues for studying rapid molecular dynamics in biological systems.