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Updated: Jan 10, 2026

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
Published on: June 25, 2014
Elevated PEDF promotes the occurrence of diabetes mellitus via suppressing GSIS by downregulating the SNARE complex
Zhen Zhao1, Yandan Tan1, Jie Fang1
1Department of Biochemistry and Molecular Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
Abstract:
Type 2 diabetes mellitus (T2DM) is a major global public health burden. The mechanisms through which glucose-stimulated insulin secretion (GSIS) can be suppressed in individuals with obesity-derived type 2 diabetes mellitus (T2DM) have not been elucidated. Compared to normal subjects, the expression of pigment epithelium-derived factor (PEDF) is upregulated in the islets of patients with obesity and T2DM. However, the role of PEDF in β-cells is unclear. This study employed male PEDF transgenic and knockout mice to assess β-cell function through oral glucose tolerance tests and GSIS assays. Complementary cell line experiments elucidated underlying mechanisms, collectively demonstrating the role of PEDF in regulating insulin secretion. PEDF suppressed GSIS by downregulating the SNARE complex, which suppresses the PI3K/Akt signaling pathway. Thus, targeting PEDF is a potential therapeutic strategy for treating T2DM.
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