Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

3.3K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Procontractile influence of ROS, produced by NADPH oxidase, is greater during contraction induced by thromboxane A<sub>2</sub> than α<sub>1</sub>-adrenoceptor activation.

Free radical research·2026
Same author

Chronic atorvastatin treatment weakens procontractile influence of NADPH oxidase derived ROS in systemic murine arteries.

Pflugers Archiv : European journal of physiology·2026
Same author

Adaptations of the cardiovascular system in hibernating mammals.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2026
Same author

Different effects of 3-week disuse on phenotype and gene expression in calf and thigh muscles.

The Journal of physiology·2026
Same author

Basic Characteristics of Systemic Arteries of Different Organs.

Journal of vascular research·2026
Same author

The Vasomotor Impact of Cu/ZnSODs Is Higher in Arterial Smooth Muscle of Early Postnatal Rats Compared to Adult Animals.

Antioxidants (Basel, Switzerland)·2025

Related Experiment Video

Updated: Jan 10, 2026

Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice
08:02

Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice

Published on: October 19, 2013

18.9K

Decrease of Rho-kinase functional activity as potential complication of birth asphyxia in newborn vasculature.

Anastasia A Shvetsova1, Anna A Borzykh2, Dina K Gaynullina3,4

  • 1Lomonosov Moscow State University, Moscow, Russia.

Pediatric Research
|November 25, 2025
PubMed
Summary
This summary is machine-generated.

Acidosis, common in birth asphyxia, reduces Rho-kinase activity in neonatal arteries. This finding explains decreased vascular resistance and blood pressure drops during neonatal asphyxia.

More Related Videos

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model
10:32

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model

Published on: April 2, 2021

4.1K
A Swine Model of Neonatal Asphyxia
10:36

A Swine Model of Neonatal Asphyxia

Published on: October 11, 2011

14.8K

Related Experiment Videos

Last Updated: Jan 10, 2026

Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice
08:02

Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice

Published on: October 19, 2013

18.9K
Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model
10:32

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model

Published on: April 2, 2021

4.1K
A Swine Model of Neonatal Asphyxia
10:36

A Swine Model of Neonatal Asphyxia

Published on: October 11, 2011

14.8K

Area of Science:

  • Neonatal physiology
  • Vascular biology
  • Acid-base balance

Background:

  • Birth asphyxia is linked to neonatal acidosis, causing a drop in blood pressure potentially due to reduced peripheral vascular resistance.
  • Mechanisms of arterial relaxation during acidosis in the early postnatal period are not well understood.
  • This study investigates the role of Rho-kinase in regulating arterial tone under acidosis in neonates.

Purpose of the Study:

  • To test the hypothesis that Rho-kinase's contribution to arterial tone regulation is diminished during acidosis in the early postnatal period.
  • To elucidate the impact of acidosis on Rho-kinase-mediated vascular responses in neonatal arteries.

Main Methods:

  • Modeled extracellular metabolic acidosis (pH 6.8) in isolated saphenous arteries from 11-14-day-old rats.
  • Utilized wire myography, Ca2+-fluorimetry, and Western blotting to assess arterial function and Rho-kinase activity.
  • Investigated the effects of acidosis and Rho-kinase inhibition (Y27632) on methoxamine-induced contractions and intracellular calcium.

Main Results:

  • Acidosis attenuated methoxamine-induced contractions and intracellular calcium ([Ca2+]i) elevation in neonatal rat arteries.
  • Rho-kinase inhibition (Y27632) reduced arterial contractility at both normal (pH 7.4) and acidic (pH 6.8) conditions, with a more pronounced effect at pH 7.4.
  • Methoxamine-induced Rho-kinase activity (p-MYPT1 at Thr855) was significantly higher at pH 7.4 compared to pH 6.8, and Y27632 reduced this phosphorylation to similar levels at both pH values.

Conclusions:

  • Acidosis suppresses the procontractile influence of Rho-kinase in arteries during the early postnatal period.
  • These findings suggest that reduced Rho-kinase activity contributes to the vasodilation observed during neonatal acidosis.
  • Understanding these mechanisms is crucial for managing cardiovascular complications associated with birth asphyxia.