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Related Concept Videos

RNA Splicing01:32

RNA Splicing

60.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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Related Experiment Video

Updated: Jan 10, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

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Alternative Splicing And Global Transcriptome Changes Associated With LPS Stimulation In Human Peripheral Blood

Esther Chavez-Iglesias, Anatol Sucher, Nidhi Thati

    Biorxiv : the Preprint Server for Biology
    |November 26, 2025
    PubMed
    Summary
    This summary is machine-generated.

    Lipopolysaccharide (LPS) triggers immune responses, and this study reveals alternative splicing (AS) significantly impacts inflammatory pathways. AS in key immune genes and non-coding RNAs offers potential therapeutic targets for immune conditions.

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    Area of Science:

    • Immunology
    • Molecular Biology
    • Genomics

    Background:

    • Lipopolysaccharide (LPS) is a key activator of innate immunity, widely used to model inflammatory responses.
    • While transcriptional changes are known, the role of alternative splicing (AS) in LPS-induced inflammation is largely uncharacterized.

    Purpose of the Study:

    • To investigate the impact of alternative splicing on the transcriptome-wide response to LPS stimulation in human Peripheral Blood Mononuclear Cells (PBMCs).

    Main Methods:

    • Deep RNA sequencing was performed on PBMCs from healthy donors stimulated with LPS.
    • Differential gene expression and alternative splicing analyses were conducted across the transcriptome.

    Main Results:

    • Identified 490 differentially expressed genes and 46 perturbed KEGG pathways, confirming known LPS responses and revealing novel pathways.
    • Alternative splicing analysis highlighted impacts on Toll-like receptor signaling, PI3K/AKT signaling, and macrophage polarization.
    • Alternative splicing events were found in key immune genes (e.g., MyD88, TLR4) and regulatory long non-coding RNAs (e.g., MALAT1, PVT1).

    Conclusions:

    • This is the first study to characterize alternative splicing genome-wide in response to LPS in PBMCs.
    • Alternative splicing is a crucial regulatory mechanism in the inflammatory response to LPS.
    • Findings suggest AS events may represent novel therapeutic targets for immune-related diseases.