An autoradiographic study of experimental odontogenic cyst formation in the mouse

Proliferative populations of cells within the epithelial lining of developing experimental odontogenic cysts were identified by labeling with 3H-thymidine. Mouse molar teeth extracted from 10-day-old mice were transplanted subcutaneously and host animals were injected with 3H-thymidine 1 h prior to sacrifice. The cysts which developed around the crown of the transplanted teeth were prepared for autoradiographical examination. Labeling indices were high initially but decreased rapidly as the cysts enlarged. The pattern of labeling, however, bore no resemblance to the labeling of the reduced enamel epithelium from which the cysts developed. It was concluded that labeling activity reflects only the state of development and activity of the cyst and not the origin of the cyst.