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Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis
Published on: January 4, 2019
In Vitro Antioxidant and Anti-Inflammatory Effects of Platelet-Rich Plasma Powder
Vivekraj Navabalan1, Ramasamy Raveendran1
1Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India.
Background And Objective:
Platelet-rich plasma (PRP) is emerging as a new treatment modality for inflammatory conditions, like osteoarthritis and ulcers, but no definitive mechanism has been established for this effect, and our study aims to explore the antioxidant and anti-inflammatory effects of the powder form of PRP on normal cells.
Materials And Methods:
We used 3T3 fibroblast and RAW 264.7 macrophage cell lines, and all cells were pretreated with lipopolysaccharide (LPS), and diclofenac was used as a positive control for anti-inflammatory studies and ascorbic acid for antioxidant studies. We evaluated PRP's anti-inflammatory action on cell lines by checking cytokine levels (IL-1, IL-6, TNF-α, and MCP-1) and nitric oxide (NO) expression. Cytotoxicity on these cell lines was further evaluated by Hoechst and AO/EB staining. Antioxidant studies were carried out by studying reactive oxygen species (ROS) generation and by two chemical methods, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and ferric reducing power assay (FRAP) assay.
Results:
In the DPPH assay, dose-dependent inhibition of DPPH was observed, while in the FRAP assay, absorbance values of optical activity were studied at 593 nm, and dose-dependent antioxidant activity was found compared to ascorbic acid. In cytotoxicity assays, PRP did not show any morphological damage. PRP exerted anti-inflammatory action in a dose-dependent manner (12.5, 25, and 50 µg) in ELISA-based cytokine assays (IL-1, IL-6, TNF-alpha, and MCP-1) and also ROS assays.
Conclusion:
PRP powder exerts anti-inflammatory and antioxidant effects on 3T3 and RAW cell lines. Further in vivo data are needed to generate the results and develop PRP powder as a therapeutic agent in various disorders.
