The present study aimed to evaluate, in vitro, the hydrolysis of PDO monofilament threads when incubated in 4 different solutions: Group A (GA) with saline solution; Group B (GB) with non-reticulated hyaluronic acid TKN HA - MW 2% (Toskani); Group C (GC) with NCTFTM. 135 HA (Filorga) and Group D (GD) with NC 160 (PHD from Brazil). For this purpose, three filaments of 1.0 cm of PDO thread (I-thread monofilament smooth 29G/38 mm, from MedBeauty) were incubated and measured at times: 0, 7, 14, 30, 45, 60, and 63 days after the start of the experiment. The sample hydrolysis was assessed by absorbance spectrometry, optical microscopy, and scanning electron microscopy. The data were analyzed using the Kruskal-Wallis, Friedman, and Stundet-Newman-Keuls t-test using SPSS 23 (SPSS Inc., Chicago, IL, USA) and BioEstat 5.0 (Manirauá Foundation, Belém, PA, Brazil) software programs, with a significance level of 5%. The spectrometry results showed that up to 14 days, the different solutions had distinct effects on the hydrolysis of PDO threads (p=0.023). After 45 days, the sample hydrolyses did not present a significant difference between the four groups, indicating that all tested solutions had a similar effect on the PDO threads (p=0.065). After 63 days, only groups GA (p=0.029) and GD (p=0.042) threads remained non-hydrolyzed, while others were completely degraded. In conclusion, the PDO association with intradermic injectables based on non-cross-linked hyaluronic acid (HA) affects the hydrolysis of the threads over time.