Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

10.4K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
10.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The rise and fall of TRPV1-targeted analgesia in osteoarthritis: a critical appraisal.

Expert opinion on pharmacotherapy·2026
Same author

Reimagining Dental Education: Integrating Digital Technologies for a Global, Digitally Native Generation-A Perspective.

European journal of dentistry·2026
Same author

TRPA1 and TRPV1 Receptors Protect the Gastric Mucosa from Ethanol-Induced Injury: Evidence from Knockout Mice.

Cells·2026
Same author

Hypothalamic orexigenic and anorexigenic neuropeptides in the rotenone model of Parkinson's disease.

Scientific reports·2026
Same author

Contact sensitisers activate keratinocytes and induce cytotoxicity via Transient Receptor Potential Ankyrin 1 in allergic contact dermatitis.

British journal of pharmacology·2026
Same author

Age-dependent alterations of TRPA1 and urocortin 1 signaling in the Edinger-Westphal nucleus in a mouse model of Alzheimer's disease.

Scientific reports·2026

Related Experiment Video

Updated: Jan 10, 2026

Rapid Isolation of Single Cells from Mouse and Human Teeth
06:09

Rapid Isolation of Single Cells from Mouse and Human Teeth

Published on: October 28, 2021

4.2K

Identification of Optimal Decalcification Method and Tissue Preparation Protocol for RNAscope In Situ Hybridization

János Konkoly1, Árpád Kunka2,3,4, Attila Szentágotai1

  • 1Department of Pharmacology and Pharmacotherapy, University of Pécs Medical School, H-7624 Pécs, Hungary.

Dentistry Journal
|November 26, 2025
PubMed
Summary

Identifying the best decalcification methods for mouse teeth is crucial for preserving RNA integrity. ACD decalcification buffer and Morse solution were found to be optimal for RNAscope in situ hybridization of dental pulp.

Keywords:
RNAscope in situ hybridizationdecalcificationdental pulpendodonticshistology

More Related Videos

Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing
10:32

Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

Published on: June 10, 2022

2.2K
Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
08:12

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants

Published on: March 29, 2018

10.6K

Related Experiment Videos

Last Updated: Jan 10, 2026

Rapid Isolation of Single Cells from Mouse and Human Teeth
06:09

Rapid Isolation of Single Cells from Mouse and Human Teeth

Published on: October 28, 2021

4.2K
Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing
10:32

Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

Published on: June 10, 2022

2.2K
Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
08:12

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants

Published on: March 29, 2018

10.6K

Area of Science:

  • Biomedical Research
  • Molecular Biology
  • Dental Research

Background:

  • Quantitative gene expression analysis using RT-qPCR lacks spatial information.
  • RNA in situ hybridization (ISH) preserves localization but is challenging in calcified tissues like teeth due to RNA damage during decalcification.
  • RNAscope, a sensitive ISH method, has potential for dental pulp but requires optimized decalcification.

Purpose of the Study:

  • To determine the optimal decalcification process for mouse tooth samples to preserve RNA integrity for RNAscope ISH.
  • To evaluate the efficacy of different decalcification methods on RNA preservation in dental pulp.

Main Methods:

  • Five decalcification methods (EDTA, Plank-Rychlo, formic acid, ACD buffer, Morse solution) were tested on mouse tooth samples.
  • Micro-CT and HE staining assessed decalcification and tissue microstructure.
  • RNAscope ISH analyzed mRNA integrity using housekeeping genes.

Main Results:

  • All methods preserved tissue structure via HE staining.
  • Only ACD decalcification buffer and Morse solution preserved RNA integrity in mouse dental pulp.
  • RNA integrity varied based on the decalcification agent used.

Conclusions:

  • ACD decalcification buffer and Morse solution are optimal for preserving RNA integrity in mouse dental pulp for RNAscope ISH.
  • These findings facilitate future RNAscope studies on dental pulp gene expression.
  • Optimized decalcification is key for successful molecular analysis in challenging tissues.