Nutritional and Techno-Functional Properties of Ultrasound-Assisted Moringa oleifera Leaf Protein Concentrate with...

Moringa oleifera leaves are a protein-rich source containing all essential amino acids and offering high nutritional value. Ultrasound-assisted extraction (UAE) has emerged as an efficient method to improve protein recovery while enhancing the structural and functional properties of plant proteins. This study aimed to improve protein extraction from M. oleifera leaves using UAE and to characterize the nutritional composition and gel-related properties of the resulting protein concentrate. Chosen conditions were a solubilization pH of 11.68, 20 min of ultrasound treatment, and precipitation at pH 4.5, resulting in an extraction yield of 79.90% and protein content of 53.97%. Moringa oleifera leaf flour (MOF) contained 29.38% protein, 37.98% dietary fiber, and high mineral levels (1751.85 mg/100 g of calcium; 512.55 mg/100 g of magnesium). Compared with MOF, the M. oleifera protein concentrate (MOPC) showed a 21.4% increase in essential amino acids, with leucine and lysine being the most abundant. Functionally, MOPC exhibited 24.26% solubility at pH 2, complete gelation at pH 8, 58.66% emulsifying capacity with 79.52% stability at pH 10, and 21.11% foaming capacity with 94.44% stability at pH 2. The gel-forming ability was the most promising characteristic, highlighting the potential of MOPC as a natural structuring agent in gel-based food systems and functional formulations.