FGAN@PB NP Nanozyme-Based Colorimetric-Photothermal Dual-Mode Immunosensor for Malachite Green Detection

Area of Science:

• Analytical Chemistry
• Materials Science
• Food Science

Background:

• Conventional enzyme-based detection methods for contaminants like malachite green (MG) face stability issues.
• Developing robust and sensitive detection strategies is crucial for ensuring the safety of aquatic products.

Purpose of the Study:

• To develop a stable and highly sensitive dual-mode immunosensor for detecting malachite green (MG) in aquatic products.
• To utilize a Fe(Ⅲ)-gallic acid composite Prussian blue nanozyme (FGAN@PB NPs) as a stable alternative to conventional enzymes.

Main Methods:

• Fabrication of a colorimetric-photothermal dual-mode immunosensor using FGAN@PB NPs.
• Assessing the sensor's performance in colorimetric mode (IC50, linear range).
• Evaluating the sensor's performance in photothermal mode (linear range, LOD).

Main Results:

• The colorimetric mode showed an IC50 of 7.56 ng/mL and a linear range of 2.21-25.84 ng/mL.
• The photothermal mode achieved a linear range of 0.262-25.6 ng/mL with a detection limit (LOD) of 0.31 ng/mL.
• Results from both modes were consistent and correlated well with LC-MS/MS analysis.

Conclusions:

• The developed dual-mode immunosensor provides a stable and sensitive platform for MG detection.
• This method offers a promising approach for rapid, on-site screening of MG in complex aquatic product samples.
• The FGAN@PB NPs-based strategy enhances the applicability and reliability of contaminant detection in food safety.