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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

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Nanogold-Lateral Flow Assay for Ginseng DNA Differentiation.

Al-Hashim Tiffere¹, Parsa Sojoudi¹, Christopher Oberc¹

¹Department of Chemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada.

|November 26, 2025

Summary

Differentiating ginseng species like Panax ginseng is crucial for industry. A new DNA lateral flow assay using single-nucleotide polymorphism (SNP) hybridization can now distinguish ginseng DNA accurately.

Keywords:

DNA hybridization gold nanoparticles lateral flow test nitrocellulose membrane single nucleotide polymorphism spot test

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Area of Science:

Botany
Molecular Biology
Biotechnology

Background:

Accurate identification of ginseng species (e.g., Panax ginseng, Panax quinquefolius) is vital for medicinal and commercial applications.
Existing differentiation methods may be insufficient for industrial-scale quality control.

Purpose of the Study:

To develop a rapid and reliable method for distinguishing between different ginseng species.
To leverage DNA hybridization based on single-nucleotide polymorphism (SNP) for ginseng identification.

Main Methods:

Genomic DNA extraction from ginseng root samples.
Polymerase chain reaction (PCR) amplification to generate DNA fragments flanking the target SNP.
DNA hybridization using probes immobilized on a nitrocellulose membrane, visualized via a spot test and lateral flow assay.

Main Results:

Successful demonstration of a DNA spot test for ginseng identification.
Development and validation of a DNA lateral flow assay capable of differentiating ginseng species.
Detection of approximately 3 nanograms of PCR amplicons in 1 microliter of sample.

Conclusions:

Ginseng DNA differentiation is achievable using a DNA lateral flow assay.
The developed assay provides a sensitive and specific method for identifying ginseng species.
This method offers a valuable tool for industrial and commercial quality control of ginseng products.