Influence of Different Biomaterials Extracted from Autologous Blood on the Cell Migration of Stem Cells from Dental
Janet N Kirilova1, Rositsa Z Vladova1, Viktoria P Petrova1
1Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University, 1000 Sofia, Bulgaria.
Background:
This study aims to evaluate the effect of different types of platelet concentrates (autologous blood biomaterials) on the migration potential of human dental pulp stem (hDPSCs).
Materials And Methods:
Our team created a model of human dental pulp stem cells (hDPSCs). Various types of AB biomaterials were produced from blood samples from volunteers using the protocols presented: A-PRF+, Gel A-PRF+, and Solid PRF. The scratch wound healing assay was used to examine the closure of the experimental wounds on day 1 and day 14. The wound areas were quantified using Image J software. Statistical analysis was performed with the Kruskal-Wallis and Mann-Whitney U tests, as the data did not follow a normal distribution, which was confirmed by the Shapiro-Wilk test (p < 0.05).
Results:
The results demonstrate significantly faster closure of the experimental wounds on day 14 of the studied biomaterials AB: A-PRF+, Gel A-PRF+, and Solid PRF compared to the control group of cells. Gel A-PRF+ exhibited the most pronounced stimulatory effect on cell migration (p = 0.0036 vs. control), followed by Solid PRF and A-PRF+.
Conclusions:
The results indicate that autologous blood platelet concentrates stimulate the migration of hDPSCs in vitro. Gel A-PRF+ demonstrated the strongest effect, underscoring its potential clinical relevance for applications in tissue engineering.
