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Identifying Microglia and Peripheral Infiltrating Macrophages in the Injured Spinal Cords Using Flow Cytometry
Published on: June 24, 2025
632
Mogroside V Ameliorates Spinal Cord Injury by Inhibiting M1 Microglia Polarization.
Xinzhou Xie1,2, Junfeng Zhang3,4, Zhihong Liu4
1Department of Spine Surgery, Jining No.1 People's Hospital Affiliated to Shandong First Medical University & Shandong Academy of Medical Sciences, Jining, 272011, China.
Molecular Neurobiology
|November 26, 2025
Summary
Mogroside-V (Mog-V) improves spinal cord injury (SCI) motor function in mice by reducing inflammation. This natural compound inhibits pro-inflammatory macrophage and microglia polarization, decreasing key inflammatory cytokines via MAPK and NF-κB pathways.
Area of Science:
- Neuroscience
- Immunology
- Pharmacology
Background:
- Spinal cord injury (SCI) is a severe central nervous system disorder with limited treatment options.
- Traditional Chinese medicines show potential for SCI treatment, including Mogroside-V (Mog-V) from Siraitia grosvenorii.
- The therapeutic effects and mechanisms of Mog-V on SCI are not well understood.
Purpose of the Study:
- To investigate the therapeutic effects of Mogroside-V (Mog-V) on spinal cord injury (SCI) in a mouse model.
- To elucidate the underlying anti-inflammatory mechanisms of Mog-V in SCI treatment.
Main Methods:
- A spinal cord contusion model was established in C57BL/6 mice.
- Mog-V treatment effects on motor function were evaluated in vivo.
- Macrophage/microglia polarization and inflammatory cytokine levels were assessed in vitro and in vivo.
- Key protein phosphorylation in MAPK and NF-κB signaling pathways was analyzed.
Main Results:
- Mog-V significantly improved lower limb motor function in mice post-SCI.
- Mog-V inhibited pro-inflammatory macrophage/microglia polarization and reduced IL-12, IL-6, IL-1β, and TNF-α secretion.
- Mog-V suppressed M1 microglial polarization and associated cytokine release in vitro.
- Mog-V inhibited phosphorylation of P65, P38, JNK, and ERK proteins.
Conclusions:
- Mog-V demonstrates therapeutic potential for spinal cord injury (SCI) by reducing neuroinflammation.
- The mechanism involves inhibiting M1 microglia/pro-inflammatory macrophage polarization and inflammatory cytokine release.
- Mog-V acts through the mitogen-activated protein kinase (MAPK) and NF-κB signaling pathways.
