Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

9.0K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
9.0K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

16.5K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
16.5K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

9.3K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
9.3K
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

3.0K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
3.0K
Rab Cascades01:25

Rab Cascades

3.4K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
3.4K
COP Coated Vesicles00:59

COP Coated Vesicles

16.9K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
16.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distribution, cellular localization, and colocalization of several peptide neurotransmitters in the central nervous system of <i>Aplysia</i>.

Learning & memory (Cold Spring Harbor, N.Y.)·2023
Same author

α-Synuclein in the Synaptic Vesicle Liquid Phase: Active Player or Passive Bystander?

Frontiers in molecular biosciences·2022
Same author

Clathrin-mediated endocytosis cooperates with bulk endocytosis to generate vesicles.

iScience·2022
Same author

Mitochondrial dysfunction in adult midbrain dopamine neurons triggers an early immune response.

PLoS genetics·2021
Same author

Preformed Ω-profile closure and kiss-and-run mediate endocytosis and diverse endocytic modes in neuroendocrine chromaffin cells.

Neuron·2021
Same author

A latent lineage potential in resident neural stem cells enables spinal cord repair.

Science (New York, N.Y.)·2020

Related Experiment Video

Updated: Jan 10, 2026

Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles
07:48

Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles

Published on: November 10, 2021

4.8K

Actin cage for the synaptic vesicle liquid phase.

Lennart Brodin1, Oleg Shupliakov1

  • 1Department of Neuroscience, Karolinska Institutet, 17177 Stockholm, Sweden.

Cell Reports
|November 26, 2025
PubMed
Summary

A novel actin filament cage forms around synaptic vesicle clusters during neural activity. This F-actin cage is crucial for maintaining vesicle proximity to the active zone for neurotransmission.

Keywords:
CP: cell biologyCP: neuroscienceactinliquid phasesynapsesynaptic vesiclesynaptic vesicle cluster

More Related Videos

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles
10:19

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

Published on: August 25, 2022

4.1K
In vitro Reconstitution of Cytoskeletal Networks inside Phase Separated Giant Unilamellar Vesicles (GUVs)
06:34

In vitro Reconstitution of Cytoskeletal Networks inside Phase Separated Giant Unilamellar Vesicles (GUVs)

Published on: June 20, 2025

1.7K

Related Experiment Videos

Last Updated: Jan 10, 2026

Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles
07:48

Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles

Published on: November 10, 2021

4.8K
In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles
10:19

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

Published on: August 25, 2022

4.1K
In vitro Reconstitution of Cytoskeletal Networks inside Phase Separated Giant Unilamellar Vesicles (GUVs)
06:34

In vitro Reconstitution of Cytoskeletal Networks inside Phase Separated Giant Unilamellar Vesicles (GUVs)

Published on: June 20, 2025

1.7K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Synaptic Plasticity

Background:

  • Synaptic vesicles cluster at the active zone, fusing with the presynaptic membrane during activity.
  • This cluster's liquid phase organization is hypothesized to depend on synapsin.

Purpose of the Study:

  • To investigate the structural organization of synaptic vesicle clusters during activity.
  • To determine the role of actin filaments in maintaining vesicle cluster integrity at the active zone.

Main Methods:

  • Utilized the lamprey giant reticulospinal synapse model.
  • Observed synaptic activity and its effect on vesicle cluster organization.
  • Disrupted F-actin cage formation using cytochalasin D, latrunculin A, and C2 toxin.

Main Results:

  • Synaptic activity induces the formation of an F-actin cage encapsulating the synaptic vesicle cluster.
  • This actin cage grows from the periactive zone over the vesicle cluster.
  • Disruption of the F-actin cage leads to vesicle cluster detachment from the release site during stimulation.

Conclusions:

  • The F-actin cage is essential for maintaining the synaptic vesicle cluster's liquid phase at the active zone.
  • This structure plays a critical role in regulating neurotransmitter release during synaptic activity.