A host-guest mediated immunomodulatory hydrogel with sustained antibacterial activity for healing of infected
Chunxian Wang1, Pingting Liu1, Yang Liu1
1Guangdong Provincial Key Laboratory of Marine Biotechnology, Department of Biology, College of Science, Shantou University, Shantou, Guangdong 515063, PR China.
Abstract:
To solve the hinderance of infected diabetic wound regeneration and healing, an antibacterial and immunomodulatory hydrogel based on host-guest mediated was designed and prepared. Firstly, carboxymethyl chitosan-carboxymethyl cyclodextrin (CMCS-CMCD) was synthesized by chemical grafting, which was applied to load essential oils for sustained active functions. Based on the host-guest mediated effect, six out of fifteen essential oils, most of them containing phenyl groups, were screened with better antibacterial effect by molecular docking. Subsequently, clove essential oil (CEO) was proved experimentally with optimal antibacterial effect and gelation properties. Secondly, sodium alginate-dopamine hydrochloride (SA-DH) was chemically modified to improve adhesion. Then the multifunctional hydrogel (SD-SD/CEO) was further constructed by physical crosslinking with CMCS-CMCD/CEO and SA-DH, in which the grafted DH could enhance almost 6-fold of the hydrogel adhesion. The bacterial inhibition experiments of SD-SD/CEO hydrogel displayed the slow release of CEO due to the host-guest mediated effect between CMCS-CMCD and CEO, which could effectively prolong the bacterial inhibition time. The bacterial inhibition experiments of SD-SD/CEO hydrogel displayed the slow release of CEO due to the host-guest mediated effect between CMCS-CMCD and CEO, which could effectively prolong the bacterial inhibition time. Finally, SD-SD/CEO hydrogel treated infected diabetic wound in vivo. The result proved that the multifunctional hydrogel has sustained antibacterial effect during wound repair, by promoting collagen deposition and facilitating immunomodulation in wound repair.
