Structural diversity of the OPSs isolated from the LPSs of the plant pathogenic bacteria Pectobacterium parmentieri...

Pectobacterium parmentieri is a pectinolytic pathogenic bacterium causing high economic losses in plant crops. Lipopolysaccharide is one of the bacterial virulence factors that play an important role in plant colonisation and interaction with host defence systems. The chemical structure of an O-polysaccharide isolated from the lipopolysaccharide of the P. parmentieri IFB5441 was determined using NMR spectroscopy and chemical methods. The repeating unit of the O-polysaccharide was composed of three galactose residues and one residue of 5,7-diamino-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid: The chemical structure of the repeating unit of the O-polysaccharide isolated from the P. parmentieri IFB5533 was also determined revealing the presence of galactose, N-acetylgalactosamine and N-acetylmannosamine.