Implementing Point-of-Care Testing in Infectious Diseases

Point-of-care testing (POCT), particularly nucleic acid-based assays, is reshaping infectious disease diagnostics by enabling faster, decentralized decision-making. Drawing on real-world hospital deployments, we identify key operational lessons and propose a roadmap for broader implementation. Using implementation research frameworks, we highlight common barriers (workflow integration, limited stewardship, financial planning) and facilitators (institutional support, clinical engagement, training, quality assurance, and perceived quick results). We advocate for structured POCT integration in critical care, where diagnostic delays impact outcomes, and suggest embedding testing within diagnostic and antimicrobial stewardship teams. We also examine outpatient and primary care expansion, emphasizing the need for multidisciplinary organization and continuity between hospital and community settings. To guide future strategies, we outline core principles: equity, sustainability, and clinical alignment. These can inform scalable approaches to diagnostic integration across diverse health systems, including resource-limited contexts. This viewpoint offers practical, experience-based guidance to support smarter, coordinated, and stewardship-aligned deployment of POCT.