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DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jan 10, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Aptamer Engineering: Strategies for Discovering Functional Nucleic Acids for Next-Generation Diagnostics and

John V L Nguyen¹, Ahlem Meziadi¹, Cyrill Brunner²

¹Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, QC, H3A 2B4, Canada.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|November 26, 2025

Summary

Aptamer engineering methods are advancing, offering faster discovery and optimization than traditional techniques. These nucleic acid ligands show promise for diverse applications in research, diagnostics, and biosensing.

Keywords:

antibody mimetics aptasensors biosensors directed evolution in vitro selection molecular engineering nucleic acid engineering

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Last Updated: Jan 10, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Area of Science:

Biotechnology
Molecular Biology
Chemical Biology

Background:

Aptamers are nucleic acid ligands offering advantages over antibodies, such as stability and lower cost.
Current aptamer discovery methods, like SELEX, are slow and low-throughput, limiting broader application.
Growing demand for faster, tailored aptamer solutions necessitates improved discovery and engineering techniques.

Purpose of the Study:

To review recent advances in aptamer engineering methods.
To discuss both in vitro and in silico approaches for aptamer discovery and optimization.
To explore current and potential innovative applications of engineered aptamers.

Main Methods:

Review of recent literature on aptamer engineering techniques.
Discussion of in vitro (wet lab) and in silico (dry lab) methods.
Analysis of applications in research, diagnostics, and biosensing.

Main Results:

Significant progress in aptamer engineering methods has been made.
New approaches expedite aptamer discovery and optimization.
Engineered aptamers have diverse applications within and outside the lab.

Conclusions:

Advancements in aptamer engineering are overcoming limitations of traditional methods.
Engineered aptamers offer innovative solutions for various scientific and commercial applications.
Future developments promise expanded use of aptamers in diagnostics, biosensing, and analytical tools.