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Updated: Jan 10, 2026

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
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Laser-assisted microbial culturomics.

Taoran Qu1,2,3, Lothar Koch2,4,5, Rumjhum Mukherjee1,2

  • 1Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany.

Nature Communications
|November 26, 2025
PubMed
Summary
This summary is machine-generated.

A new laser-assisted culturomics platform enables rapid microbial isolation from biofilms. This method significantly improves the recovery of species for detailed microbiome research and clinical applications.

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Area of Science:

  • Microbiology
  • Biotechnology
  • Genomics

Background:

  • Metagenomics revolutionized human microbiome characterization but lacks detailed mechanistic studies due to insufficient culture collections.
  • Robust microbial culture collections are essential for advancing microbiome research beyond identification.

Purpose of the Study:

  • To develop and validate a laser-assisted culturomics platform for efficient microbial isolation and characterization from clinical biofilms.
  • To enable the creation of on-demand culture collections for research and clinical use.

Main Methods:

  • Development of a laser-assisted bioprinter platform integrating culture conditions, microbial characterization methods, and a biobank.
  • Rapid and precise transfer of microorganisms from clinical biofilms to organized microbial colony arrays for co-culturing and molecular analysis.
  • Utilizing microscopy, spectroscopy, and enzyme assays to guide microbial isolation processes.

Main Results:

  • Successfully propagated 99% of microbial species and recovered 79% of abundant species from dental plaque.
  • Generated 249 representative isolates from oral biofilms across 14 classes and 124 species.
  • Functional profiling identified commensals potentially contributing to disease and characterized peri-implantitis isolates.

Conclusions:

  • The laser-assisted culturomics platform significantly enhances microbial isolation efficiency from complex biofilms.
  • This technology provides a foundation for generating custom culture collections and biofilms for research and clinical applications.
  • Facilitates deeper mechanistic studies of the human microbiome and its role in health and disease.