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Updated: Jan 10, 2026
Author Spotlight: Standardizing and Improving the Extraction and Purification of Extracellular Vesicles from Human ADSCs
Published on: May 3, 2024
Donor-dependent heterogeneity in therapeutic effects of adipose tissue extracellular vesicles
Danielle J Beetler1,2,3, Presley Giresi2, Vivian Xu2
1Center for Clinical and Translational Science, Mayo Clinic, Rochester, MN, USA.
Background:
Extracellular vesicles (EVs) are promising therapeutics for diseases associated with inflammation and tissue remodeling. However, a major limitation for the clinical translation of EV therapeutics is heterogeneity, which is donor dependent. In this study we sought to assess the physiochemical characteristics and therapeutic efficacy of tissue-derived EVs from different donors. Donor-dependent therapeutic effects of cell culture and biofluid-derived EVs have previously been shown, but remains largely unknown for tissue-derived EVs. We obtained EV-enriched samples from various sources of adipose tissue and examined their effect in reducing inflammation in a highly translational model of myocarditis.
Results:
We demonstrate that the molecular composition of EVs varies depending on the donor and that therapeutic efficiency is donor-dependent even when controlling for age and sex.
Conclusions:
Our findings indicate that further research is needed to identify critical donor characteristics that predict therapeutic ability of individual or pooled adipose tissue-derived EVs to reduce inflammation and fibrosis.
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