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Protein kinase D2-mediated maintenance complex component 2 phosphorylation promotes bladder cancer progression
He Tian1, Jing Song1, Junzi Cong2
1Department of Urinary Surgery, Hongqi Hospital Affiliated to Mudanjiang Medical University, No. 5 Tongxiang Road, Aimin District, Mudanjiang, 157011, Heilongjiang, China.
Journal of Translational Medicine
|November 27, 2025
Summary
Protein kinase D2 (PRKD2) is highly expressed in bladder cancer, promoting tumor growth and metastasis. Inhibiting PRKD2 hinders cancer cell progression, suggesting PRKD2 as a potential therapeutic target for bladder cancer treatment.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Bladder cancer is a prevalent malignancy driven by aberrant signaling pathways.
- Protein kinase D2 (PRKD2) plays a significant role in various human cancers.
Purpose of the Study:
- To investigate the role and mechanism of PRKD2 in bladder cancer.
- To determine if PRKD2 is a potential therapeutic target for bladder cancer.
Main Methods:
- Quantitative real-time PCR, Western blotting, and tissue microarrays analyzed PRKD2 expression.
- In vitro and in vivo functional assays assessed the impact of PRKD2.
- Site-directed mutagenesis and immunoprecipitation identified PRKD2's phosphorylation target.
Main Results:
- PRKD2 expression is significantly elevated in bladder cancer tissues.
- Higher PRKD2 levels correlate with metastasis, advanced TNM stage, and poor prognosis.
- PRKD2 depletion suppressed bladder cancer cell proliferation, migration, invasion, and epithelial-mesenchymal transition.
- PRKD2 phosphorylates MCM2 at serine 139, driving cancer progression.
Conclusions:
- PRKD2 contributes to bladder cancer malignancy through MCM2 phosphorylation.
- PRKD2 represents a potential therapeutic target for bladder cancer.
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