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Horizontal Gene Transfer01:27

Horizontal Gene Transfer

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Horizontal gene transfer (HGT) is a process where genetic material moves between organisms within the same generation, unlike vertical gene transfer, which occurs from parent to offspring. HGT plays a crucial role in microbial evolution, adaptation, and survival, particularly in shared environments like the human gut.Mobile genetic elements such as plasmids, prophages, integrons, insertion sequences, and transposons facilitate this process. HGT occurs through three primary mechanisms:...
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Types of Genetic Transfer Between Organisms02:18

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Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
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Factors Influencing Microbial Growth: Temperature01:27

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Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...
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Transformation01:26

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Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
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Transduction01:16

Transduction

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Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
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Diversity of Archaea III01:27

Diversity of Archaea III

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Crenarchaeota, a prominent phylum of Archaea, is remarkable for its ability to thrive in extreme environments characterized by high temperatures and acidity. These microorganisms inhabit sulfuric hot springs, volcanic systems, and submarine hydrothermal vents, where temperatures often exceed 100°C. The unique adaptations of Crenarchaeota not only allow survival under such extreme conditions but also provide insights into the mechanisms of life in primordial Earth-like...
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Updated: Jan 10, 2026

Establishment of Microbial Eukaryotic Enrichment Cultures from a Chemically Stratified Antarctic Lake and Assessment of Carbon Fixation Potential
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Cold adaptation and horizontal gene transfer shape Antarctic sponge microbiomes.

Maria F Manrique-de-la-Cuba1, Marileyxis López-Rodríguez1, Sebastián Abades1

  • 1GEMA Center for Genomics, Ecology & Environment, Universidad Mayor, Santiago, Chile.

Microbiome
|November 27, 2025
PubMed
Summary

Antarctic sponge microbiomes possess unique cold-adaptation genes, with horizontal gene transfer (HGT) playing a key role. This study reveals how HGT shapes microbial symbioses in extreme polar environments.

Keywords:
AdaptationAntarcticaColdHorizontal gene transferMAGMetagenomicsSponge microbiomeSponge symbiont

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Area of Science:

  • Marine Microbiology
  • Ecology
  • Evolutionary Biology

Background:

  • Marine sponges host diverse, vital microbiomes across various global environments.
  • Antarctic sponges harbor unique microbial communities adapted to extreme cold.
  • Functional differences in Antarctic sponge microbiomes remain largely unexplored.

Purpose of the Study:

  • Investigate functional differences in Antarctic sponge microbiomes compared to other environments.
  • Focus on cold adaptation functions within these microbiomes.
  • Assess the role of horizontal gene transfer (HGT) in driving functional adaptations.

Main Methods:

  • Comparative analysis of functional gene content in Antarctic, temperate, and tropical sponge microbiomes.
  • Identification of genes associated with cold adaptation.
  • Assessment of horizontal gene transfer prevalence and mechanisms (e.g., conjugation, ICEs).

Main Results:

  • Antarctic sponge microbiomes show higher proportions of cold adaptation genes (e.g., cold shock proteins, chaperones, osmoprotectants).
  • Horizontal gene transfer (HGT) is prevalent in Antarctic sponge symbionts, contributing to metabolic functions and cold adaptation.
  • The cold shock protein C (CspC) was identified as a potential horizontally acquired gene exclusive to Antarctic sponge symbionts.

Conclusions:

  • Antarctic sponge microbiomes exhibit enhanced cold adaptation through functional genes facilitated by HGT.
  • HGT mechanisms are evolutionarily significant in shaping microbial symbioses in extreme environments.
  • Further research into HGT dynamics and specific symbionts can illuminate microbial evolution and host-symbiont interactions in polar ecosystems.