Related Experiment Video
Updated: Jan 10, 2026
10:27
In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
Published on: October 21, 2022
1.9K
Molecular Characteristics, Circularization Mechanism, and Potential Functions of circRNA-0172
Haojie Wang1, Wenhao Li1, Ting Yang1
1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Genes
|November 27, 2025
Summary
This study identifies a novel circular RNA, circRNA-0172, derived from MDM2, which is downregulated by Avian Leukosis Virus Subgroup J (ALV-J). Its dysregulation impacts cell proliferation and apoptosis, offering potential therapeutic targets for ALV-J-induced avian leukemia.
Area of Science:
- Molecular Biology
- Virology
- Poultry Science
Background:
- Avian leukosis virus subgroup J (ALV-J) causes significant economic losses in poultry through tumors and immunosuppression.
- The role of MDM2-derived circular RNA-0172 (circRNA-0172) in ALV-J infection has not been previously elucidated.
Purpose of the Study:
- To investigate the molecular characteristics, circularization mechanism, and functional role of circRNA-0172 in the context of ALV-J infection.
- To explore circRNA-0172 as a potential target for controlling ALV-J-associated diseases in poultry.
Main Methods:
- Cell culture (CEF/DF-1/HD11) and nucleic acid extraction.
- Validation of circRNA-0172 using PCR, sequencing, and RNase R treatment; analysis of subcellular localization and circularization.
- Bioinformatic analysis (miRanda, TargetScan, GO, KEGG) and functional assays (CCK-8, qRT-PCR) to assess targets, pathways, and cellular functions.
Main Results:
- circRNA-0172, a stable, cytoplasm-localized molecule formed via flanking repeat elements, was identified and characterized.
- ALV-J infection led to the downregulation of circRNA-0172 in immune tissues and HD11 cells.
- Overexpression of circRNA-0172 inhibited DF-1 cell proliferation, modulated Bcl2 and CCND1 expression, suggesting roles in cell cycle regulation and apoptosis.
Conclusions:
- This study provides the first evidence of circRNA-0172's stability and repeat-dependent formation.
- circRNA-0172 plays a regulatory role in cell proliferation and apoptosis.
- circRNA-0172 represents a promising molecular target for developing strategies against ALV-J-associated avian leukemia.
Related Concept Videos
01:19RNA Structure
7.0K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
7.0K
01:23RNA Structure
78.7K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
78.7K
00:58Eukaryotic RNA Polymerases
26.6K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
26.6K
00:58Eukaryotic RNA Polymerases
9.0K
9.0K
00:55DNA Helicases
23.8K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
23.8K
00:43Bacterial RNA Polymerase
32.4K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
32.4K