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Impact of CCD Inhibition on Semi-Quantitative Multiple Allergen Simultaneous Test
Hyeyoung Lee1, Kyungcheol Song1, Jiwoo Kim1
1Department of Laboratory Medicine, International St. Mary's Hospital, College of Medicine, Catholic Kwandong University, Incheon 22711, Republic of Korea.
Diagnostics (Basel, Switzerland)
|November 27, 2025
Summary
Cross-reactive carbohydrate determinants (CCDs) cause false positives in allergy tests. Inhibiting CCDs significantly reduced these false positives in multiple allergen simultaneous testing (MAST), improving accuracy for food and pollen allergies.
Area of Science:
- Allergy and Immunology
- Diagnostic Testing
- Biochemistry
Background:
- Cross-reactive carbohydrate determinants (CCDs) are known to cause false-positive results in allergen-specific IgE assays.
- This can lead to an overestimation of polysensitization, complicating accurate allergy diagnosis.
Purpose of the Study:
- To evaluate the impact of CCD inhibition on semi-quantitative multiple allergen simultaneous testing (MAST).
- To assess the effectiveness of a CCD-blocker in reducing false-positive IgE results.
Main Methods:
- Analysis of 65 serum samples with anti-CCD IgE.
- Comparison of 6624 allergen-specific IgE results before and after CCD-blocker pretreatment.
- Dose-response analysis with varying CCD-blocker concentrations.
Main Results:
- CCD inhibition converted 64.1% of positive IgE results to negative.
- Significant reductions in IgE positivity were observed for food (28.2% to 8.1%) and pollen (70.1% to 23.3%) allergens.
- Venom allergens like honey bee and yellow jacket showed significant IgE level decreases, resolving double-positivity in 94.1% of cases.
Conclusions:
- CCD inhibition markedly improves the specificity of MAST by reducing false-positive results.
- The findings highlight the importance of CCD blocking for accurate diagnosis, especially for food and pollen allergies.
