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Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

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Published on: June 14, 2013

Ecological History Shapes Transcriptome Variation in Quiescent Saccharomyces cerevisiae.

Agnieszka Marek¹, Katarzyna Tomala², Dominika Wloch-Salamon²

¹Institute of Botany, Faculty of Biology, Jagiellonian University, 30-387 Kraków, Poland.

|November 27, 2025

Summary

Cellular quiescence, a state of suspended cell division, shows variability. Yeast cells adapt their gene activity based on evolutionary history, impacting energy, biosynthesis, and cell wall maintenance.

Keywords:

RNA-seq life history quiescent cells

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Published on: October 22, 2018

Area of Science:

Cell Biology
Microbiology
Evolutionary Biology

Background:

Cellular quiescence is a fundamental state across organisms, crucial for survival.
Recent findings highlight heterogeneity within quiescent cell populations, despite shared division potential.
Entry into quiescence is linked to nutrient deprivation and time, but mechanisms require further study.

Purpose of the Study:

To investigate transcriptomic differences in quiescent yeast cells from diverse ecological histories.
To understand how evolutionary adaptation influences cellular characteristics during quiescence.

Main Methods:

Isolation of homogenous fractions of dense quiescent yeast.
Comparison of transcriptomes from yeast populations with distinct experimental enrichment histories (300 generations).

Main Results:

Transcriptome analysis revealed significant variations in quiescent yeast.
Discrepancies were observed in genes related to energy metabolism, biosynthesis, and cell wall synthesis.
These variations correlate with the ecological histories of the yeast populations.

Conclusions:

Quiescent cells exhibit adaptive plasticity at the transcriptomic level.
Evolutionary history shapes the molecular characteristics of quiescent cells.
This suggests a dynamic adaptation of cellular states beyond simple dormancy.