Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Discovery: Overview01:26

Drug Discovery: Overview

10.9K
Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
10.9K
Structure-Activity Relationships and Drug Design01:28

Structure-Activity Relationships and Drug Design

1.7K
Drug design is a dynamic field that involves discovering and developing new medications based on specific biological targets. This process heavily relies on structure-activity relationships (SAR) and quantitative structure-activity relationships (QSAR) to guide the design and optimization of efficient drugs.
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence...
1.7K
Biopharmaceutical Factors Influencing Drug Product Design: Overview01:22

Biopharmaceutical Factors Influencing Drug Product Design: Overview

222
Rational drug product design integrates knowledge of the drug’s physicochemical properties, formulation components, manufacturing techniques, and intended route of administration. Each factor influences the drug’s performance, including how it is released, absorbed, and eliminated in the body.The physicochemical properties of a drug—such as solubility, stability, and particle size—affect its compatibility with excipients and the choice of dosage form. Excipients, though...
222
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.4K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.4K
Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

603
Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
603
Biopharmaceutics and Pharmacokinetics: Overview01:28

Biopharmaceutics and Pharmacokinetics: Overview

3.2K
Understanding drugs, drug products, and their performance in pharmaceutical science is pivotal. Drugs, whether simple molecules or complex compounds, are designed to interact with the body's biological systems to diagnose, treat, or prevent diseases. Drug products include various delivery systems such as tablets, capsules, injections, and inhalers. The performance of these drug products is gauged by their ability to deliver the active ingredient to the desired site of action at the...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adherent-Invasive <i>Escherichia coli</i> (AIEC) in Crohn's Disease: A Bibliometric Analysis of 25 Years of Research (1999-2025).

Microorganisms·2026
Same author

Antibacterial and antibiofilm evaluation of biogenic ternary CuO-AgO-MgO nanocomposite against clinical isolates of carbapenem-resistant <i>Klebsiella pneumoniae</i>.

Artificial cells, nanomedicine, and biotechnology·2026
Same author

Exploring the Multifunctional Benefits of Astaxanthin in Aging, Oxidative Stress, Immune Dysfunction, Gut and Skin Health.

Antioxidants (Basel, Switzerland)·2026
Same author

Sulforaphane-Activated Functional Nucleic Acids for Cancer Therapy: Mechanisms, Delivery Strategies, and Nanomedicine Advances.

International journal of molecular sciences·2026
Same author

Epigenetic Alterations in Microbiome-Host Interactions in Inflammatory and Autoimmune Diseases.

International journal of molecular sciences·2026
Same author

<i>Bacteroides thetaiotaomicron</i> (<i>BT6</i>) Restores Intestinal Homeostasis in <i>Escherichia coli</i> O157:H7-Challenged Mice.

Veterinary sciences·2026

Related Experiment Video

Updated: Jan 10, 2026

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis
08:49

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis

Published on: June 20, 2025

1.1K

Protein Engineering and Drug Discovery: Importance, Methodologies, Challenges, and Prospects.

Ahmed Mohammed1, Nasir A Ibrahim2, Nosiba S Basher2

  • 1Department of Biotechnology, College of Life Sciences and Technology, Omdurman Islamic University, Omdurman 382, Sudan.

Biomolecules
|November 27, 2025
PubMed
Summary

Protein engineering advances drug discovery by creating targeted biologics like antibodies. Overcoming challenges like stability and immunogenicity with new technologies promises improved patient outcomes.

Keywords:
CRISPR-CasVHH domainsbiologicsdrug discoverymonoclonal antibodiesprotein engineering

More Related Videos

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.5K
Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
05:08

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins

Published on: July 8, 2025

909

Related Experiment Videos

Last Updated: Jan 10, 2026

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis
08:49

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis

Published on: June 20, 2025

1.1K
Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.5K
Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
05:08

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins

Published on: July 8, 2025

909

Area of Science:

  • Biotechnology and Pharmaceutical Sciences
  • Molecular Biology and Biochemistry

Background:

  • Protein engineering is crucial for developing novel therapeutics.
  • Engineered proteins offer enhanced specificity and reduced side effects compared to small molecules.
  • Challenges include protein folding, stability, immunogenicity, and regulatory hurdles.

Purpose of the Study:

  • To review the impact of protein engineering on drug discovery and development.
  • To highlight advancements in protein design methodologies and technologies.
  • To discuss challenges and future directions in recombinant protein-based therapies.

Main Methods:

  • Review of current literature on protein engineering applications in medicine.
  • Analysis of technologies such as phage display, yeast display, CRISPR, and computational modeling.
  • Examination of case studies on approved recombinant protein therapies.

Main Results:

  • Engineered proteins, including monoclonal antibodies and therapeutic enzymes, show significant promise in treating complex diseases.
  • Technological integration enhances the predictability and efficiency of protein design.
  • Successful development and approval of recombinant protein therapies demonstrate the field's maturity.

Conclusions:

  • Protein engineering is transforming medicine by enabling the development of highly specific and effective biologics.
  • Addressing challenges through technological innovation is key to unlocking new therapeutic possibilities.
  • The field holds immense potential for improving patient outcomes and revolutionizing healthcare.