Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same authorSame journal

Precision Proteomic Profiling of Systemic Lupus Erythematosus-Correlating Disease Activity and Complement Levels with Clinical Phenotypes.

Biomedicines·2026

Same author

Application of Hi-C sequencing to detect oncogene rearrangements for diagnosis and treatment of large B-cell lymphoma.

Blood advances·2026

Same author

Toward Precision Health in Autoimmunity and Immune-Related Adverse Events: The Autoantibody Reactome, Spatial Omics, and Multimodal Data Integration.

Biomedicines·2026

Same author

Bridging the Precision Gap in Rheumatoid Arthritis: Spatial Transcriptomics, Spatial Proteomics, and Artificial Intelligence in Precision Health.

Biomedicines·2026

Same author

Epcoritamab plus rituximab, dexamethasone, cytarabine, oxaliplatin/carboplatin induces deep and durable responses in transplant-eligible patients with relapsed or refractory diffuse large B-cell lymphoma: results from the EPCORE NHL-2 trial.

Haematologica·2026

Same author

The Association of right atrium and sinoatrial node irradiation with atrial fibrillation and radiation-induced heart disease in non-small cell lung cancer.

Acta oncologica (Stockholm, Sweden)·2026

Search research articles

Related Experiment Video

Updated: Jan 10, 2026

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

Proteomic Study of Diffuse Large B-Cell Lymphoma Identifying Proteins Associated with R-CHOP Response.

Hulda Haraldsdóttir^1,2,3, Rasmus Froberg Brøndum^3,4, Marie Hairing Enemark^5,6

¹Department of Hematology, Aalborg University Hospital, 9000 Aalborg, Denmark.

|November 27, 2025

Summary

Researchers identified 16 proteins linked to treatment response in diffuse large B-cell lymphoma (DLBCL). These proteins, including GET4 and NSFL1C, could serve as biomarkers for predicting patient outcomes and guiding therapy for relapsed/refractory DLBCL.

Keywords:

R-CHOP diffuse large B-cell lymphoma prognostic markers proteomics

More Related Videos

Enhancing Tumor Content through Tumor Macrodissection

Enhancing Tumor Content through Tumor Macrodissection

Published on: February 12, 2022

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

Published on: June 10, 2017

Related Experiment Videos

Last Updated: Jan 10, 2026

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

Enhancing Tumor Content through Tumor Macrodissection

Enhancing Tumor Content through Tumor Macrodissection

Published on: February 12, 2022

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

Published on: June 10, 2017

Area of Science:

Oncology
Proteomics
Molecular Biology

Background:

Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous cancer with variable responses to R-CHOP therapy.
A significant portion of DLBCL patients develop relapsed/refractory disease (rrDLBCL), necessitating better prognostic and diagnostic biomarkers.
The molecular underpinnings of rrDLBCL remain incompletely understood.

Purpose of the Study:

To identify novel protein biomarkers associated with treatment response in DLBCL.
To explore the proteomic differences between drug-sensitive and drug-resistant DLBCL cell lines.
To validate potential biomarkers in patient cohorts for clinical relevance.

Main Methods:

Proteomic profiling of DLBCL cell lines with varying sensitivity to R-CHOP components.
Comparative analysis of protein abundance between resistant and sensitive cell lines.
Validation of differentially expressed proteins in independent DLBCL patient cohorts.

Main Results:

98 differentially abundant proteins were identified between resistant and sensitive DLBCL cells.
16 proteins showed consistent association with treatment response in both cell lines and patient samples.
GET4 and NSFL1C were significantly enriched in R-CHOP resistant patients and linked to cyclophosphamide and vincristine resistance, respectively.
An enrichment of DNA damage repair proteins suggests their involvement in treatment resistance.

Conclusions:

16 proteins demonstrate concordant specificity for treatment response in DLBCL cell lines and patient tissues.
These proteins hold potential as prognostic markers for stratifying DLBCL patients.
Further investigation into DNA damage repair pathways is warranted for understanding treatment responses.