Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Self-organizing bioceramic granules with wave-dissipating architectures for bone void filling.

Biomaterials·2026
Same author

Three-dimensionally-printed biphasic PCL/<b>β</b>-TCP scaffold with spatially confined GelMA/CS hydrogel for coordinated osteochondral regeneration.

Regenerative biomaterials·2026
Same author

A Mechanically Adaptive Titanium Scaffold With a Lattice-Modulated Piezocatalytic Coating for Infection Treatment and Bone Regeneration.

Advanced healthcare materials·2026
Same author

Zinc-containing biomaterials for bone disease therapy and tissue repair: Design principles, mechanistic insights, and translational pathways.

Biomaterials·2026
Same author

Melanin Building Block as Scaffold for Dynamic Submicromolar Galectin Inhibitors.

ACS omega·2026
Same author

A Straightforward Access to Sustainable and Reusable Melanin-Supported Palladium Catalysts: Characterization and Application in Sonogashira Cross-Coupling Reactions.

ACS applied materials & interfaces·2026

Related Experiment Video

Updated: Jan 10, 2026

Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting
07:40

Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting

Published on: March 31, 2022

3.1K

In Vitro Osteogenic Stimulation of Human Adipose-Derived MSCs on Biofunctional 3D-Printed Scaffolds.

Serena Munaò1, Ugo D'Amora2, Luana Vittoria Bauso1

  • 1Department of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.

Biomedicines
|November 27, 2025
PubMed
Summary

Adding bioactive factors like eumelanin and hydroxyapatite to methacrylated gellan gum scaffolds significantly enhances bone regeneration in vitro. These modified scaffolds show improved osteogenic differentiation for bone tissue engineering applications.

Keywords:
bioactive factorsbone regenerationmesenchymal stem cellsnatural polymer-based hydrogelsosteoconductive and osteoinductive properties

More Related Videos

Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation
05:42

Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation

Published on: April 5, 2024

1.4K
Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs
10:19

Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs

Published on: August 8, 2022

2.4K

Related Experiment Videos

Last Updated: Jan 10, 2026

Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting
07:40

Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting

Published on: March 31, 2022

3.1K
Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation
05:42

Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation

Published on: April 5, 2024

1.4K
Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs
10:19

Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs

Published on: August 8, 2022

2.4K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Human adipose-derived mesenchymal stem cells (hADMSCs) are crucial for regenerative medicine due to their proliferative and differentiation capabilities.
  • Bone tissue engineering offers an alternative to traditional grafts using biomaterials, stem cells, and bioactive factors.
  • Gellan gum (GG) is biocompatible but requires methacrylation (GGMA) for improved mechanical properties and stability in scaffolds.

Purpose of the Study:

  • To evaluate the biological response of osteoprogenitor cells cultured on three-dimensional (3D) printed GGMA-based scaffolds.
  • To compare neat GGMA scaffolds with those functionalized with hydroxyapatite (HAp) and black soldier fly-derived eumelanin (BSF-Eumelanin).
  • To assess the potential of incorporating bioactive factors into GGMA scaffolds for bone regeneration.

Main Methods:

  • Cell adhesion, viability, and proliferation were assessed using MTT assays and histological staining (H&E).
  • Osteogenic differentiation was evaluated through Alizarin Red S staining, alkaline phosphatase (ALP) activity assays, and gene expression analysis of osteogenic markers.
  • In vitro cell culture was conducted over 21 days on the different GGMA scaffold types.

Main Results:

  • All tested GGMA-based scaffolds supported cell adhesion, growth, and proliferation.
  • Scaffolds functionalized with BSF-Eumelanin and HAp demonstrated significantly enhanced osteogenic differentiation compared to neat GGMA.
  • The incorporation of bioactive factors positively influenced cell behavior on the scaffolds.

Conclusions:

  • Embedding bioactive factors like BSF-Eumelanin and HAp into GGMA scaffolds improves their osteoconductive and osteoinductive potential.
  • Modified GGMA scaffolds present a promising strategy for enhancing bone repair and regeneration.
  • This approach offers a viable alternative for developing advanced bone graft substitutes.