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Transcriptomic Profiling and Histological Validation of Preputial Fibrosis in Hypospadias
Yaping Wang1, Zhiwei Peng1, Yu Ding1
1Department of Urology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200062, China.
Biomedicines
|November 27, 2025
Summary
Hypospadias is linked to abnormal inner prepuce development, characterized by a hyper-fibrotic state. This finding may impact wound healing and complications after surgery for hypospadias.
Area of Science:
- Urology
- Developmental Biology
- Genetics
Background:
- Hypospadias, a congenital condition, is frequently associated with abnormal development of the prepuce.
- Understanding the molecular and histological differences in the inner prepuce of hypospadias patients is crucial for identifying causes and developing targeted treatments.
Purpose of the Study:
- To investigate transcriptomic and histological differences in the inner prepuce between hypospadias patients and controls.
- To identify molecular pathways and histological features associated with abnormal prepuce development in hypospadias.
Main Methods:
- Transcriptomic sequencing of dorsal and ventral inner preputial tissues from hypospadias patients and children with phimosis (controls).
- Bioinformatic enrichment analysis of differentially expressed genes.
- Histological staining to validate transcriptomic findings and assess tissue characteristics.
Main Results:
- Significant differences in gene expression were observed in the inner prepuce of hypospadias patients compared to controls.
- A substantial number of upregulated genes were related to fibrosis, indicating a hyper-fibrotic state.
- Histological analysis confirmed increased collagen fibers, altered collagen type ratio (I/III), and reduced microvessel density in the dorsal inner prepuce of hypospadias patients.
Conclusions:
- The inner prepuce in hypospadias patients exhibits a hyper-fibrotic state.
- This fibrotic condition may have significant implications for post-operative wound healing and the occurrence of complications following hypospadias repair.
