Solid Phase Extraction and Determination of Tetracycline Using Gold Nanoparticles Stabilized in a Polymethacrylate...

Area of Science:

• Analytical Chemistry
• Materials Science

Background:

• Tetracycline (TC) contamination is a growing concern in environmental and food safety.
• Existing methods for TC detection often involve complex extraction and elution steps.

Purpose of the Study:

• To develop a simplified solid-phase extraction (SPE) method for tetracycline (TC) determination.
• To investigate the use of a polymethacrylate matrix (PMM) for direct TC analysis.

Main Methods:

• Solid-phase extraction using a polymethacrylate matrix (PMM) and PMM-Au0.
• Direct determination of TC via intrinsic absorption and fluorescence spectroscopy.
• Application of digital colorimetry for quantitative analysis.

Main Results:

• Optimized extraction of TC as a singly charged anion (H2TC-) in the pH range of 8.9-9.7.
• Achieved low detection limits for TC: 0.03 mg/L (absorption) and 0.001 mg/L (fluorescence).
• Demonstrated successful application in analyzing water, biological fluids, honey, and milk samples.

Conclusions:

• PMM provides an efficient matrix for direct solid-phase extraction and determination of tetracycline.
• The developed method simplifies TC analysis, offering high sensitivity and broad applicability.
• This approach holds promise for routine monitoring of tetracycline in diverse matrices.