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Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line
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Dual Roles of CD147 in Regulating THP-1 Monocyte Migration and MCP-1-Induced Inflammatory Responses.

Nutjeera Intasai1,2, Kanokporn Sornsuwan2,3, On-Anong Juntit2,3

  • 1Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.

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Cluster of Differentiation (CD) 147 knockout monocytes show increased migration and altered inflammatory cytokine production. This suggests CD147 regulates monocyte chemotaxis and inflammatory balance.

Keywords:
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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Cluster of Differentiation (CD) 147 is a transmembrane glycoprotein crucial for monocyte functions like invasion, migration, and cytokine production.
  • Monocyte chemoattractant protein-1 (MCP-1) is a key chemokine involved in monocyte recruitment and inflammatory responses.

Purpose of the Study:

  • To investigate the role of CD147 in regulating monocyte chemotaxis and inflammatory responses.
  • To analyze the effects of CD147 knockout (CD147KO) on THP-1 monocyte migration and cytokine profiles in response to MCP-1.

Main Methods:

  • Utilized CD147 knockout (CD147KO) THP-1 monocytes and wild-type (WT) THP-1 cells.
  • Assessed monocyte migration towards MCP-1 and breast cancer cell-secreted chemoattractants.
  • Quantified surface expression of CD44, CC chemokine receptor type 1 (CCR1), and CC chemokine receptor type 2 (CCR2).
  • Measured mRNA expression of key inflammatory cytokines (IL-6, IL-10, TNF-α) following MCP-1 stimulation.

Main Results:

  • CD147KO THP-1 cells demonstrated significantly enhanced migration compared to WT cells.
  • Surface expression of CD44, CCR1, and CCR2 remained unchanged in CD147KO cells.
  • MCP-1 stimulation led to elevated IL-6 and IL-10 mRNA expression and reduced TNF-α mRNA expression in CD147KO monocytes.
  • IL-6 upregulation was identified as a potential mediator of enhanced monocyte migration.

Conclusions:

  • CD147 acts as a regulatory checkpoint for THP-1 monocyte migration, with its function being context-dependent.
  • CD147 knockout influences the balance between pro-inflammatory and anti-inflammatory cytokine responses in monocytes.
  • These findings provide insights into the complex role of CD147 in immune cell behavior and inflammatory processes.