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Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

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Exosomal Protein Markers as Potential Non-Invasive Biomarkers for Colorectal Cancer.

Maciej Skrzypek¹, Danuta Piotrzkowska¹, Julia Adamkiewicz²

¹Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, 92-215 Lodz, Poland.

International Journal of Molecular Sciences

|November 27, 2025

Summary

Size exclusion chromatography (SEC) effectively isolates exosomes from plasma for colorectal cancer (CRC) detection. Elevated HSP70, CK19, and CA125 in patient exosomes show promise for non-invasive CRC diagnosis.

Keywords:

biomarkers colorectal cancer exosome isolation methods exosomes size exclusion chromatography ultracentrifugation

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Published on: May 27, 2016

Area of Science:

Biochemistry
Molecular Biology
Oncology

Background:

Exosomes, small extracellular vesicles in body fluids, carry biomolecules reflecting cellular states.
These characteristics make exosomes potential biomarkers for diseases like colorectal cancer (CRC).

Purpose of the Study:

To evaluate and compare plasma exosome isolation methods for CRC diagnostics.
To identify potential exosomal protein biomarkers for non-invasive CRC detection.

Main Methods:

Compared ultracentrifugation (UC), size exclusion chromatography (SEC), and a commercial kit for exosome isolation.
Assessed isolated exosome purity, protein content, and integrity.
Utilized ELISA to quantify CRC-related proteins (HSP70, CK19, CA125, TAG72) in exosomes.

Main Results:

SEC was selected as the optimal isolation method based on purity and integrity.
Significantly higher levels of HSP70, CK19, and CA125 were found in CRC patient exosomes compared to controls.
TAG72 levels did not significantly differ between groups.
HSP70 levels were higher in male patients than in female patients.

Conclusions:

SEC is a viable method for isolating plasma exosomes for biomarker analysis.
HSP70, CK19, and CA125 show potential as non-invasive biomarkers for colorectal cancer diagnosis and monitoring.