Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Molecular Targets in Alveolar Rhabdomyosarcoma: A Narrative Review of Progress and Pitfalls.

International journal of molecular sciences·2025

Same author

Effect of photobiomodulation therapy on the morphology, intracellular calcium concentration, free radical generation, apoptosis and necrosis of human mesenchymal stem cells-an in vitro study.

Lasers in medical science·2024

Same author

There and back again: a dendrimer's tale.

Drug and chemical toxicology·2021

Same author

Neurotoxicity of poly(propylene imine) glycodendrimers.

Drug and chemical toxicology·2020

Search research articles

Related Experiment Video

Updated: Jan 10, 2026

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

Published on: April 6, 2016

Advances in Cytotoxicity Testing: From In Vitro Assays to In Silico Models.

Barbara Ziemba¹

¹Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland.

International Journal of Molecular Sciences

|November 27, 2025

Summary

Modern toxicology is advancing beyond basic cell viability assays. New Approach Methodologies (NAMs) integrate computational toxicology and advanced in vitro models for more predictive and mechanistically informed safety assessments.

Keywords:

3D cell culture cytotoxicity assays high-content imaging in silico models integrated testing strategies (IATA)organ-on-a-chip toxicity testing

More Related Videos

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

Published on: February 27, 2019

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

Related Experiment Videos

Last Updated: Jan 10, 2026

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

Published on: April 6, 2016

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

Published on: February 27, 2019

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

Area of Science:

Toxicology
Cell Biology
Computational Science

Background:

Classical cytotoxicity assays (MTT, LDH, neutral red) are foundational but lack mechanistic depth and physiological relevance.
The field is shifting towards human-centered approaches for more predictive toxicology.
Limitations in traditional methods necessitate the development of advanced testing strategies.

Purpose of the Study:

To explore the evolution of cytotoxicity testing from classical assays to advanced methodologies.
To highlight the integration of computational toxicology with novel in vitro models.
To emphasize the development of predictive, mechanism-anchored frameworks for safety assessment.

Main Methods:

High-content imaging, flow cytometry, and real-time impedance analysis for multiparametric cytotoxicity assessment.
In silico models including Quantitative Structure-Activity Relationships (QSAR), machine learning, and physiologically based pharmacokinetic (PBPK) modeling.
Integration of computational tools with 3D organoids, organ-on-chip systems, and stem cell-based models.

Main Results:

Advanced methods enable detection of adaptive and sub-lethal cellular responses.
In silico models facilitate quantitative in vitro-in vivo extrapolation (QIVIVE).
Integration of computational and experimental approaches enhances mechanistic interpretation and translational accuracy.

Conclusions:

New Approach Methodologies (NAMs) and Integrated Approaches to Testing and Assessment (IATA) represent a paradigm shift in toxicology.
These frameworks bridge in silico predictions with in vitro and in vivo validation for enhanced accuracy.
The evolution advances both biomedical research and regulatory toxicology by providing predictive, mechanism-anchored safety evaluations.