Related Experiment Video
Updated: Jan 10, 2026

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
Published on: January 21, 2016
Neobacillus terrisolis sp. nov. and Neobacillus solisequens sp. nov. Isolated from Soil
Haoyu Wu1,2, Congguo Ran2,3, Nan Zhou2
1State Key Laboratory of Geological Processes and Mineral Resources, China University of Geosciences, Beijing 100083, China.
Abstract:
Two bacterial strains, designated LXY-1T and LXY-4T, were isolated from soil samples collected at a heavy metal smelting plant located in Guangxi, China. Phylogenetic analysis indicated that these strains formed two distinct lineages within the genus Neobacillus. Both strains were characterized as facultative anaerobic, Gram-positive staining, endospore-forming, non-motile, short-rod bacteria. The major cellular fatty acids identified in these strains included C16:0, iso-C15:0, antéiso-C15:0, and antéiso-C17:0. The predominant polar lipids comprised diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), and phosphatidylglycerol (PG). The average nucleotide identity (ANI) values between the newly isolated strains and their closest phylogenetic relatives, the type strains of the genus Neobacillus, were found to be below 95%, with corresponding digital DNA-DNA hybridization (dDDH) values remaining below 70%. Based on a comprehensive polyphasic taxonomic analysis incorporating chemotaxonomic, phenotypic, phylogenetic, and genomic data, we proposed that strains LXY-1T and LXY-4T represent two novel species of the genus Neobacillus, for which the names Neobacillus terrisolis sp. nov. and Neobacillus solisequens sp. nov. are designated. The type strains are LXY-1T (= CGMCC 30313T = JCM 37671T) and LXY-4T (= CGMCC 1.62901T = JCM 37672T), respectively.
More Related Videos
Related Concept Videos
Anoxygenic Phototrophic Bacteria
Bacterial Phylum Bacteroidota
Other Unique Bacteria
Bacterial Phylum Tenericutes
Bacterial Phylum Actinobacteria
The Roles of Bacteria and Fungi in Plant Nutrition

