Targeted Biocatalyst Design for Asymmetric Citalopram Conversion in a Membrane Reactor.

Area of Science:

• Biocatalysis and Enzyme Engineering
• Materials Science
• Organic Chemistry

Background:

• Enzyme immobilization is crucial for developing stable and reusable biocatalysts.
• Metal-organic frameworks (MOFs) offer unique properties for supporting enzyme immobilization.
• Ionic liquids can enhance enzyme stability and activity.

Purpose of the Study:

• To develop a stable and active biocatalytic system for enzyme immobilization.
• To utilize an electrospun support doped with MOF and an ionic liquid for lipase stabilization.
• To apply the system for efficient and enantioselective resolution of racemic citalopram.

Main Methods:

• Enzyme immobilization on electrospun supports doped with MOF and ionic liquid.
• Optimization of MOF concentration, electrospinning, immobilization conditions, and ionic liquid selection.
• Application in the enantioselective resolution of racemic citalopram.

Main Results:

• Optimal immobilization achieved 52% efficiency and 100% activity recovery within 24 hours.
• The biocatalytic system (HIGH PVC-MOF-lip-CA) showed 80% retained activity after storage, a 75% improvement over free enzyme.
• Achieved 96% conversion of S-citalopram with 93% enantiomeric excess in 24 hours.

Conclusions:

• The developed biocatalytic system demonstrates enhanced stability and activity for enzyme immobilization.
• The system is effective for efficient and stereoselective biocatalytic applications, specifically in citalopram resolution.
• This approach holds potential for advancing biocatalysis in pharmaceutical and chemical synthesis.