Related Experiment Video
Updated: Jun 23, 2026
11:32
Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
Published on: November 12, 2013
11.8K
9-Methylfascaplysin, a Marine-Derived Bioactive Compound, Promotes Neurite Outgrowth via the Inhibition of ROCK2
Meilin Zheng1, Kangyang Gao1, Yirui Hong1
1Translational Medicine Center of Pain, Emotion and Cognition, Health Science Center, Ningbo University, Ningbo 315211, China.
Pharmaceuticals (Basel, Switzerland)
|November 27, 2025
Summary
Marine compound 9-Methylfascaplysin (9-MF) promotes neurite outgrowth by inhibiting Rho-associated coiled-coil containing protein kinase 2 (ROCK2). This suggests 9-MF
Area of Science:
- Neuroscience
- Pharmacology
- Marine Biotechnology
Background:
- Neurite outgrowth impairment is key in neurodegenerative diseases.
- Promoting neurite outgrowth offers a therapeutic strategy.
- 9-Methylfascaplysin (9-MF), a marine compound, shows neuroprotective potential.
Purpose of the Study:
- To investigate 9-MF's effect on neurite outgrowth.
- To elucidate the molecular mechanism of 9-MF's neuroprotective action.
Main Methods:
- PC12 cell culture and treatment with 9-MF.
- Gene expression analysis (CMap).
- Molecular docking and protein kinase assays.
Main Results:
- 9-MF promoted neurite outgrowth and GAP-43 expression in PC12 cells.
- 9-MF associated gene expression linked to mitochondria and synapse, centered on ROCK2.
- 9-MF demonstrated high binding affinity to ROCK2 and reduced its phosphorylation.
Conclusions:
- 9-MF promotes neurite outgrowth, potentially by inhibiting ROCK2.
- 9-MF shows promise as a novel therapeutic agent for neurological disorders.
Related Concept Videos
01:19Actin Filament Depolymerization
Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
In F-actin, the ADF/cofilin proteins...
In F-actin, the ADF/cofilin proteins...
01:39Mechanism of Filopodia Formation
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...