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Bridging the Bio-Electronic Interface with Biofabrication

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A ZIF-8-Based High-Performance Glucose Electrochemical Detection Platform Constructed Using a Multi-Layer Interface

Canjie Hu¹, Pengjia Qi^1,2, Lichao Liu³

¹School of Information Science and Engineering (School of Cyber Science and Technology), Zhejiang Sci-Tech University, Hangzhou 310018, China.

Sensors (Basel, Switzerland)

|November 27, 2025

Summary

This summary is machine-generated.

This study presents a novel electrochemical glucose biosensor using MWCNTs/PB/ZIF-8@GOx/CS for rapid and accurate glucose detection. The advanced sensor offers high sensitivity and stability, showing great promise for clinical diagnostics and food testing.

Keywords:

Gox MWCNTs ZIF-8 electrochemical biosensor glucose detection

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Area of Science:

Electrochemistry
Biosensors
Materials Science

Background:

Accurate glucose determination is crucial for clinical diagnostics and food safety.
Existing methods face challenges in speed, accuracy, and stability.
Development of novel biosensors is needed to meet these demands.

Purpose of the Study:

To develop a high-performance electrochemical glucose biosensor.
To utilize multi-walled carbon nanotubes/Prussian blue/zeolitic imidazolate framework-8@glucose oxidase/chitosan (MWCNTs/PB/ZIF-8@GOx/CS) for enhanced glucose detection.
To evaluate the sensor's performance characteristics for practical applications.

Main Methods:

Fabrication of a composite material incorporating MWCNTs, PB, ZIF-8, GOx, and CS.
Electrochemical characterization of the fabricated biosensor.
Assessment of sensing performance, including linear range, sensitivity, detection limit, repeatability, and stability.

Main Results:

The MWCNTs/PB conductive network enhanced electron transfer and catalytic activity.
ZIF-8 provided an optimal microenvironment for GOx immobilization, improving enzyme activity and substrate diffusion.
The MWCNTs/PB/ZIF-8@GOx/CS sensor exhibited a wide linear response (4.8 μM–2.24 mM), high sensitivity (579.57 μA/mM/cm²), low detection limit (0.55 μM), excellent repeatability (RSD=1.49%), and good storage stability (86.23% after 3 weeks).

Conclusions:

The developed electrochemical glucose biosensor demonstrates superior performance for glucose detection.
The synergistic integration of MWCNTs, PB, ZIF-8, GOx, and CS contributes to the enhanced sensing capabilities.
The sensor shows significant potential for practical applications in clinical diagnostics and food testing due to its accuracy, sensitivity, and stability.