A ZIF-8-Based High-Performance Glucose Electrochemical Detection Platform Constructed Using a Multi-Layer Interface...

Area of Science:

• Electrochemistry
• Biosensors
• Materials Science

Background:

• Accurate glucose determination is crucial for clinical diagnostics and food safety.
• Existing methods face challenges in speed, accuracy, and stability.
• Development of novel biosensors is needed to meet these demands.

Purpose of the Study:

• To develop a high-performance electrochemical glucose biosensor.
• To utilize multi-walled carbon nanotubes/Prussian blue/zeolitic imidazolate framework-8@glucose oxidase/chitosan (MWCNTs/PB/ZIF-8@GOx/CS) for enhanced glucose detection.
• To evaluate the sensor's performance characteristics for practical applications.

Main Methods:

• Fabrication of a composite material incorporating MWCNTs, PB, ZIF-8, GOx, and CS.
• Electrochemical characterization of the fabricated biosensor.
• Assessment of sensing performance, including linear range, sensitivity, detection limit, repeatability, and stability.

Main Results:

• The MWCNTs/PB conductive network enhanced electron transfer and catalytic activity.
• ZIF-8 provided an optimal microenvironment for GOx immobilization, improving enzyme activity and substrate diffusion.
• The MWCNTs/PB/ZIF-8@GOx/CS sensor exhibited a wide linear response (4.8 μM–2.24 mM), high sensitivity (579.57 μA/mM/cm²), low detection limit (0.55 μM), excellent repeatability (RSD=1.49%), and good storage stability (86.23% after 3 weeks).

Conclusions:

• The developed electrochemical glucose biosensor demonstrates superior performance for glucose detection.
• The synergistic integration of MWCNTs, PB, ZIF-8, GOx, and CS contributes to the enhanced sensing capabilities.
• The sensor shows significant potential for practical applications in clinical diagnostics and food testing due to its accuracy, sensitivity, and stability.