Related Experiment Video
Updated: Jan 10, 2026

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool
Published on: May 27, 2016
The relationship between the expression and clinicopathology of long non-coding RNA in older adults with non-small
Huimin Liu1, Zhiqing Feng1, Qi Quan2
1Oncology Department 2, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China.
Abstract:
This study investigates the expression of long non-coding RNA (lncRNA) in older adults with non-small cell lung cancer (NSCLC) and its relationship with clinicopathology. Tumor and adjacent normal tissues were collected from 97 elderly NSCLC patients. The differentiation antagonizing non-protein coding RNA (DANCR) expression was analyzed using microarray and validated by quantitative reverse transcription polymerase chain reaction. Bioinformatic methods identified DANCR-associated pathways. Clinicopathological correlations were assessed using logistic regression, and survival analysis was performed based on 24-month follow-up data. DANCR was significantly upregulated in tumor tissues (P < .005) and associated with larger tumor diameter, advanced stage, and poor differentiation (P < .05). Patients with low DANCR expression exhibited longer overall survival and progression-free survival (P < .05). lncRNAs are differently expressed and potentially play a regulatory role in the occurrence and development of NSCLC in older adults. Candidate lncRNA DANCR is highly expressed in older adults with NSCLC, which closely relates to clinical pathology and can contribute to progression-free survival and overall survival time prediction.
Related Concept Videos
lncRNA - Long Non-coding RNAs
lncRNA - Long Non-coding RNAs
Non-LTR Retrotransposons
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
The Nucleolus